{"product_id":"roti-block-potein-free-a151-a151","title":"ROTI®Block, potein free, ready-to-use, 10x conc., 250 ml, glass","description":"\u003cp\u003eROTI®Block, potein free, ready-to-use, 10x conc., Density (D) ~1,086 g\/cm³, Boiling point (bp) ~100 °C, Storage temp. +15 to +25 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eProtein-free blocking solution for all blot and hybridisation systems. Diluted also perfectly suitable as base solution for immunochemical detections.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eSuitable for:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eWestern- and Far Western Analysis\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSouthern- and Northern-Blots\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eDot-Blots\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eELISA- and Sandwich-Assays\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003ePrecoating of ELISA-plates (incl. drying)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\n\u003ci\u003ein situ\u003c\/i\u003e hybridisation\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMicroarray hybridisation\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eIn order to obtain high signal intensity, nonspecific antibody binding sites must be blocked. Whilst detergents are not effective as blocking agents, the addition of proteins such as BSA may mask specific binding sites.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eBlock is an innovative, polymer-based blocking reagent which solves such problems. With ROTI\u003csup\u003e®\u003c\/sup\u003eBlock, the specific binding sites remain accessible while nonspecific reactions are suppressed, thus leading to an increase in signal intensity.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eBlock is a protein-free blocking solution that can be used diluted as a base solution for all steps of Western detections and ELISAs. ROTI\u003csup\u003e®\u003c\/sup\u003eBlock replaces PBS\/TBS buffers and other buffer systems including the blocking reagents used.If required, ROTI\u003csup\u003e®\u003c\/sup\u003eBlock can be enriched with further blocking reagents, detergents etc. to further optimise the detection system.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure:\u003c\/b\u003e Western blotting of decreasing amounts (50 ng - 17 pg) of a bacterial extract including a 40 kDa protein. Probed by Anti His Serum (1:1.000) and Carl ROTH Anti Rabbit-HRP, blocking by means of ROTI\u003csup\u003e®\u003c\/sup\u003eBlock. Detection via chemoluminescence.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eIncluded you will find a detailed instruction manual with protocols, e.g. for stripping ROTI\u003csup\u003e®\u003c\/sup\u003ePVDF-membranes from bound probes without destabilising the blocking, which enables fast rescreening of the filter. ROTI\u003csup\u003e®\u003c\/sup\u003eBlock also enables the blot to be Ponceau stained even after blocking.For detection systems using antibodies specifically directed against protein-phosphorylations we recommend to test and optimise the blocking efficiency prior to assaying your samples.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-A151-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wyMTMyMDJ8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmhoT1M5b1pXWXZPVEF6TnpjeU9EVTBOamcwTmk1d1pHWXwxYjQwYTBlZTg4MDgyMDY1ZjJiMTFmMTJhNGZiMDc2NTQwNjU3NjMzYjNjZjdiMmYyODA1NWFjMzdmYjlhM2Ni\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"250 ml \/ 1 piece","offer_id":56173603389772,"sku":"A151.1","price":67.65,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 6+ pieces","offer_id":56173603422540,"sku":"A151.1","price":64.27,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 24+ pieces","offer_id":56173603455308,"sku":"A151.1","price":60.89,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 1 piece","offer_id":56173603488076,"sku":"A151.4","price":117.2,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 6+ pieces","offer_id":56173603520844,"sku":"A151.4","price":111.34,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 24+ pieces","offer_id":56173603553612,"sku":"A151.4","price":105.48,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 1 piece","offer_id":56173603586380,"sku":"A151.2","price":203.2,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 6+ pieces","offer_id":56173603619148,"sku":"A151.2","price":203.2,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 24+ pieces","offer_id":56173603651916,"sku":"A151.2","price":203.2,"currency_code":"EUR","in_stock":true},{"title":"2.5 liter \/ 1 piece","offer_id":56173603684684,"sku":"A151.3","price":385.95,"currency_code":"EUR","in_stock":true},{"title":"2.5 liter \/ 6+ pieces","offer_id":56173603717452,"sku":"A151.3","price":366.65,"currency_code":"EUR","in_stock":true},{"title":"2.5 liter \/ 24+ pieces","offer_id":56173603750220,"sku":"A151.3","price":347.36,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/A151-1000Wx1000H_09697e67-d3ac-4808-9dee-e382567fae25.jpg?v=1766444201","url":"https:\/\/www.luminixhealth.com\/de-be\/products\/roti-block-potein-free-a151-a151","provider":"Luminix Health","version":"1.0","type":"link"}