{"product_id":"roti-cell-trypsin-edta-solution-1x-1y1a-1y1a","title":"ROTI®Cell Trypsine\/EDTA-oplossing (1x), steriel, 1x, CELLPURE®, 100 ml, kunststof","description":"\u003cp\u003eROTI®Cell Trypsine\/EDTA-oplossing (1x), steriel, 1x, CELLPURE®, Dichtheid (D) 1 g\/cm³, Opslagtemp. -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\u003cdiv\u003e\u003cb\u003eSteriel\u003c\/b\u003e\u003c\/div\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"details-container\"\u003e\n\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eTrypsine is een eiwitsplitsend spijsverteringsenzym uit de pancreas dat behoort tot de serineproteasen en dat lysine en arginine aan de C-terminus splitst. Trypsine-oplossingen worden gebruikt voor het scheiden van cellen uit weefsel of celmonolagen. Ze zijn daarom essentieel voor routinematige toepassingen bij het passeren van celculturen, voor celkweekexperimenten om een adequaat aantal cellen te verkrijgen of voor de dissociatie van primair weefsel. Als alternatief voor trypsine kan de celvriendelijkere oplossing ROTI\u003csup\u003e®\u003c\/sup\u003eCell Accutase-oplossing worden gebruikt, die vrij is van componenten van zoogdieren.\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\n\u003c\/div\u003e\n\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"details-container\"\u003e\n\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eDeze trypsine-oplossing bevat EDTA. EDTA is een chelaatvormer en verbetert het vermogen van trypsine om adherente cellen los te maken. EDTA bindt calcium en magnesium, wat de celcontacten verder verzwakt. Dit bevordert de hydrolyse van specifieke peptidebindingen door trypsine.\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\n\u003c\/div\u003e\n\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"details-container\"\u003e\n\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eDe benodigde trypsineconcentratie hangt primair af van het celtype en de leeftijd van de celcultuur. Ter oriëntatie vindt u een volledig protocol in de brochure met technische informatie.\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\n\u003c\/div\u003e\n\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"details-container\"\u003e\n\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eNa celdissociatie moet trypsine worden geïnhibeerd. Dit kan worden gedaan door gebruik te maken van FCS of trypsine-inhibitoren.\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\n\u003c\/div\u003e\n\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\n\u003cdiv class=\"product-classifications\"\u003e\n\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnische informatie\u003c\/div\u003e\n\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\n\u003ctbody\u003e\n\n\u003ctr\u003e\n\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eEnzym\u003c\/td\u003e\n\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\n\t\t\t\t\t\t\t\t\tTrypsine uit pancreas van varkens \u003c\/td\u003e\n\n\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAlternatieve naam\u003c\/td\u003e\n\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\n\t\t\t\t\t\t\t\t\tCeldissociatiereagentia \u003c\/td\u003e\n\n\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eGebruik\u003c\/td\u003e\n\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\n\t\t\t\t\t\t\t\t\tCeldissociatie \u003c\/td\u003e\n\n\n\u003c\/tr\u003e\n\n\u003ctr\u003e\n\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eGebruiksaanwijzing, toevoeging\u003c\/td\u003e\n\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\n\t\t\t\t\t\t\t\t\tBereiding van aliquots aanbevolen om herhaalde vries-dooi-cycli te vermijden. \u003c\/td\u003e\n\n\n\u003c\/tr\u003e\n\n\n\u003c\/tbody\u003e\n\n\n\u003c\/table\u003e\n\n\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem; 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align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    CE-conformiteitsverklaring (PDF)\n  \u003c\/a\u003e\n\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"100 ml \/ 1 stuk","offer_id":56127477743948,"sku":"1Y1A.1","price":18.2,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ stuks","offer_id":56127477776716,"sku":"1Y1A.1","price":17.29,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ stuks","offer_id":56127477809484,"sku":"1Y1A.1","price":16.38,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1Y1A-1000Wx1000H_5b0966b3-e29c-4711-8b55-77c12f46158a.jpg?v=1766408281","url":"https:\/\/www.luminixhealth.com\/de-be\/products\/roti-cell-trypsine-edta-oplossing-1x-1y1a-1y1a","provider":"Luminix Health","version":"1.0","type":"link"}