{"product_id":"roti-free-strippingpuffer-2-2-plus-3337","title":"ROTI®Free StrippingPuffer 2.2 plus, kant-en-klaar, 250 ml, glas","description":"\u003cp\u003eROTI®Free Stripping-Puffer 2.2 plus, klaar voor gebruik, dichtheid (D) 1,01 g\/cm³, kookpunt (bp) ~100 °C, opslagtemp. +15 tot +25 °C, UN-nr. 1760\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"details-container\"\u003e\n\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eStripping-buffer voor het verwijderen van high-affinity antilichamen en chemiluminescentiekleuringen van Western blot-membranen. Door de lage pH van de buffer wordt de conformatie van het antilichaam veranderd, waardoor alle aan de epitopen gebonden moleculen voorzichtig worden verwijderd (Legocki \u003ci\u003eet. al.\u003c\/i\u003e (1981) \u003ci\u003eAnalyt. Biochem.\u003c\/i\u003e 111:385-92). Speciaal ontworpen additieven verhogen de efficiëntie bij het verwijderen van die antilichamen waarvoor bij standaard stripping-buffersystemen lange strippingtijden of hoge incubatietemperaturen vereist zijn. Geavanceerde formulering zonder β-mercaptoethanol of DTT voor gebruik bij kamertemperatuur of verwarmd. Geschikt voor nitrocellulose- en PVDF-membranen.\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"feature-container\"\u003e\n\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eZeer effectief\u003c\/li\u003e\n\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMild\u003c\/li\u003e\n\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGeurvrij\u003c\/li\u003e\n\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eEenvoudige toepassing\u003c\/li\u003e\n\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOok geschikt voor high-affinity antilichamen\u003c\/li\u003e\n\n\n\u003c\/ul\u003e\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c\/div\u003e\n\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cdiv class=\"paragraph-title\"\u003eGebruiksaanwijzing\u003c\/div\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eNa spoelen in PBST- of TBST-buffer wordt het membraan 30-60 minuten bij kamertemperatuur in ROTI\u003csup\u003e®\u003c\/sup\u003eFree Stripping-Puffer 2.2 plus geïncubeerd. In het geval van zeer hardnekkige antilichamen is incubatie in een verwarmde buffer toegestaan. Na opnieuw wassen kan het membraan worden geblokkeerd en opnieuw worden geanalyseerd. De specifieke tijd die nodig is voor volledige stripping hangt af van de betreffende antilichaam\/epitoop-combinatie.\u003c\/p\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\n\u003c\/div\u003e\n\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\n\u003c\/div\u003e\n\n\n\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem; display:flex; flex-direction:column; align-items:flex-start; gap:0.55rem;\"\u003e\n\n  \u003ca href=\"https:\/\/api.carlroth.com\/medias\/SPEZ-3337-EN.pdf?context=bWFzdGVyfHNwZWNpZmljYXRpb25zfDk1ODQ2fGFwcGxpY2F0aW9uL3BkZnxhR1k1TDJoaVlpODVNalkzTkRRNE1qRTNOak13TDFOUVJWcGZNek16TjE5RlRpNXdaR1l8YWFjZGEyYjFlYTg1ZTE4ZWVjMjBhNTRhZWY1MGY2YTA3MDRkZTU4MDExM2NiZTYwNjAyM2U5ODU2YzZmMjhiMA\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technische documentatie (PDF)\n  \u003c\/a\u003e\n  \u003ca href=\"https:\/\/api.carlroth.com\/medias\/SDB-3337-DE-EN.pdf?context=bWFzdGVyfHNlY3VyaXR5RGF0YXNoZWV0c3wzMTk3NTF8YXBwbGljYXRpb24vcGRmfGFHRTNMMmd6TlM4NU1qQTJOamN4TURFMU9UWTJMMU5FUWw4ek16TTNYMFJGWDBWT0xuQmtaZ3w5YzBmMzgwYTdhNGI5ZjJlMDEwNmM4YjExMjc0NjFmZmQxNjQwMzAwMzkwMDYyNjgwODllOWNlNDIxYjY0OWI1\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\u003c\/svg\u003e\n    Veiligheidsinformatieblad (PDF)\n  \u003c\/a\u003e\n\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"250 ml \/ 1 stuk","offer_id":56149207417164,"sku":"3337.1","price":66.55,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 6+ stuks","offer_id":56149207449932,"sku":"3337.1","price":63.22,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 24+ stuks","offer_id":56149207482700,"sku":"3337.1","price":59.9,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 1 stuk","offer_id":56149207515468,"sku":"3337.2","price":160.2,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 6+ stuks","offer_id":56149207548236,"sku":"3337.2","price":160.2,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 24+ stuks","offer_id":56149207581004,"sku":"3337.2","price":160.2,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/3337-1000Wx1000H_c240a40a-2ba2-4623-a605-bf4987ed2a6a.jpg?v=1766434354","url":"https:\/\/www.luminixhealth.com\/de-be\/products\/roti-vrij-stripping-buffer-2-2-plus-3337","provider":"Luminix Health","version":"1.0","type":"link"}