{"product_id":"roti-garose-his-ni-hpbeads-plus-0806","title":"ROTI®Garose-His\/Ni HPBeads plus, for biochemistry, 25 ml, plastic","description":"\u003cp\u003eROTI®Garose-His\/Ni HPBeads plus, for biochemistry, Density (D) 0,85 to 0,95 g\/cm³, Flash point (flp) \u0026gt;50 °C, Boiling point (bp) \u0026gt;80 °C, Storage temp. +4 °C, UN-Nr. 1170\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFor the isolation of very high yields of pure His-tagged proteins under non-reducing conditions.Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.ROTI\u003csup\u003e®\u003c\/sup\u003eGarose His\/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuperior recovery rate of pure His-tagged proteins\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVery low Nickel leaching\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCompatible with denaturing reagents\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe matrix of ROTI\u003csup\u003e®\u003c\/sup\u003eGarose His\/Ni products consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni\u003csup\u003e2+\u003c\/sup\u003e charged matrix the first choice for all standard applications. ROTI\u003csup\u003e®\u003c\/sup\u003eGarose His\/Ni beads give very high yields of pure His-tagged protein with considerably low metal contamination.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI\u003csup\u003e®\u003c\/sup\u003eGarose Beads may repeatedly be regenerated, making them very cost-effective.The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).A special feature is the modified polychelate linker used in the ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni HPBeads plus (0806). For more detailed data please see this product.For insulation under reducing conditions we recommend ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni HPBeads plus or ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni NTA beads.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eNickel charged agarose beads for affinity chromatography under presence of EDTA or DTT. The matrix of choice for applications under low or medium pressure, and applicable for every required sample and\/or matrix volume.In ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni HPBeads \u003ci\u003eplus\u003c\/i\u003e, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate. The \u003cb\u003especially developed polychelating ligand\u003c\/b\u003e forms highly stabile nickel chelates, which are able to isolate His-tagged proteins even from solutions containing 20 mM EDTA or DTT - reliably and without undesired nickel stripping.50 % bead suspension in 20 % ethanol.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRapid one-step purification of very pure His-tagged proteins from total lysates \u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh binding capacity for 6xHis-tagged protein (≥80 mg\/ml purified His-tagged protein)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eReliably elution and regeneration\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMinimized Nickel leaching\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eCompatible with 20 mM EDTA and DTT\u003c\/b\u003e\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eFor batch mode, gravity flow, MPLC und FPLC\u003c\/b\u003e\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eRecommended for all matrix volumes\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eSuitable for\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eMPLC and FPLC, or if His-tag proteins shall be isolated either\u003cb\u003e in particularly short time or in big amounts from reducing solutions\u003c\/b\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eIn the matrix of ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni HPBeads \u003ci\u003eplus,\u003c\/i\u003e IDA was replaced by a polychelator conjugation. This multi dentate cross-linker binds his-tagged proteins very efficiently, leading to high recovery rates with minimized nickel bleeding into the eluate. ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni HPBeads \u003ci\u003eplus\u003c\/i\u003e may not be regenerated.The matrix is very stabile and may generally be used with buffers including the following reagents: ≤20 mM EDTA, ≤20 mM DTT, ≤8 M urea, ≤6 M guanidinium hydrochloride, ≤100 % methanol, ≤100 % ethanol, ≤30 % acetonitrile, pH range 4-9. May be autoclaved at 121 °C for 30 mins.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eInstructions for use, addition\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tBead suspension, binding capacity ≥80 mg\/ml packed matrix (His)\u003csub\u003e6\u003c\/sub\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-0806-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w0OTM5NDF8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmd5WlM5b05EZ3ZPVEEwTURjMU9EZzVPRGN4T0M1d1pHWXw5ZThlMDllNGFlOTMxMDM3NDY0ODdlMWNjYzE0ZTU0YmE3MWIyNTZkYzZiMDk3ODg2NTU3YzI4MDNiNDRkM2Zm\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"25 ml \/ 1 piece","offer_id":56112599466316,"sku":"0806.1","price":805.2,"currency_code":"EUR","in_stock":true},{"title":"25 ml \/ 6+ pieces","offer_id":56112599499084,"sku":"0806.1","price":764.94,"currency_code":"EUR","in_stock":true},{"title":"25 ml \/ 24+ pieces","offer_id":56112599531852,"sku":"0806.1","price":724.68,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 1 piece","offer_id":56112599564620,"sku":"0806.2","price":2784.25,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ pieces","offer_id":56112599597388,"sku":"0806.2","price":2645.04,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ pieces","offer_id":56112599630156,"sku":"0806.2","price":2505.83,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/0806-1000Wx1000H.jpg?v=1766399056","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/roti-garose-his-ni-hpbeads-plus-0806","provider":"Luminix Health","version":"1.0","type":"link"}