{"product_id":"roti-garose-his-ni-nta-cartridges-0804","title":"ROTI®Garose-His\/Ni NTA-Cartridges, for biochemistry, 1 unit(s), plastic","description":"\u003cp\u003eROTI®Garose-His\/Ni NTA-Cartridges, for biochemistry, Density (D) 0,85 to 0,95 g\/cm³, Flash point (flp) \u0026gt;50 °C, Boiling point (bp) \u0026gt;80 °C, Storage temp. +4 °C, UN-Nr. 1170\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFor the isolation of His-tagged proteins under reducing conditions.Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.ROTI\u003csup\u003e®\u003c\/sup\u003eGarose His\/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuperior recovery rate of pure His-tagged proteins\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMinimized Nickel leaching\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eCompatible with denaturing and reducing reagents\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe matrix of ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni\u003csup\u003e2+\u003c\/sup\u003e charged matrix the first choice for all standard applications. The tetradentate NTA crosslinker enables highly efficient binding of His-tagged proteins and leads to high recovery rates with minimized nickel bleeding into the eluate.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni NTA-Beads may be regenerated, making them very cost-effective.The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10  mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003ePrepacked cartridges with Nickel charged NTA-agarose bead matrix for medium pressure affinity chromatography (MPLC) under reducing conditions.Immobilized metal ion affinity chromatography (IMAC) under medium pressure is the prefect method to obtain high yield of very pure proteins in minimum time. ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni NTA-Cartridges offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates in reducing buffer solutions.Matrix in 15 % ethanol.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVery fast and simple purification of His-tagged proteins from total lysates \u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh binding capacity for 6×His-tagged protein\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRecommended flow rate 5 ml\/min\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eStandard ports 10-32 (1\/16″)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVery versatile. Applicable with ÄKTA\u003csup\u003e™\u003c\/sup\u003eFPLC\u003csup\u003e™\u003c\/sup\u003e, pump and syringe\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eReliable elution and regeneration\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eFor medium pressure flow chromatography\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eSuitable for\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eÄKTA\u003c\/b\u003e™FPLC™ and for sample application via peristaltic pump or syringe.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eGarose His\/Ni NTA-Cartridges are prepacked and may be used directly.Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1\/16″) female, outlet port 10-32 (1\/16″) male.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eInstructions for use, addition\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tPrepacked columns, binding capacity 50-250 mg\/column (His)\u003csub\u003e6\u003c\/sub\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-0804-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w3MzQxMDF8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmczTmk5b01XWXZPVEEwTURjMU9EZ3dNRFF4TkM1d1pHWXxjNzAzZWJlMDU0YjM0Zjk4MzZlZGIwMzQxODA0ZDEwYjk0MzMwNTdiMjAzMGM2ZDM4ZjE0ZDhjZTYwMTVjYWEz\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 pc(s) \/ 1 piece","offer_id":56112598909260,"sku":"0804.1","price":299.95,"currency_code":"EUR","in_stock":true},{"title":"1 pc(s) \/ 6+ pieces","offer_id":56112598942028,"sku":"0804.1","price":299.95,"currency_code":"EUR","in_stock":true},{"title":"1 pc(s) \/ 24+ pieces","offer_id":56112598974796,"sku":"0804.1","price":299.95,"currency_code":"EUR","in_stock":true},{"title":"5 pc(s) \/ 1 piece","offer_id":56112599007564,"sku":"0804.2","price":1084.7,"currency_code":"EUR","in_stock":true},{"title":"5 pc(s) \/ 6+ pieces","offer_id":56112599040332,"sku":"0804.2","price":1030.47,"currency_code":"EUR","in_stock":true},{"title":"5 pc(s) \/ 24+ pieces","offer_id":56112599073100,"sku":"0804.2","price":976.23,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/0804-1000Wx1000H.jpg?v=1766399054","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/roti-garose-his-ni-nta-cartridges-0804","provider":"Luminix Health","version":"1.0","type":"link"}