{"product_id":"roti-garose-his-ni-nta-hpbeads-0805","title":"ROTI®Garose-His\/Ni NTA-HPBeads, for biochemistry, 25 ml, plastic","description":"\u003cp\u003eROTI®Garose-His\/Ni NTA-HPBeads, for biochemistry, Density (D) 0,85 to 0,95 g\/cm³, Flash point (flp) \u0026gt;50 °C, Boiling point (bp) \u0026gt;80 °C, Storage temp. +4 °C, UN-Nr. 1170\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFor the isolation of His-tagged proteins under reducing conditions.Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.ROTI\u003csup\u003e®\u003c\/sup\u003eGarose His\/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuperior recovery rate of pure His-tagged proteins\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMinimized Nickel leaching\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eCompatible with denaturing and reducing reagents\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe matrix of ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni\u003csup\u003e2+\u003c\/sup\u003e charged matrix the first choice for all standard applications. The tetradentate NTA crosslinker enables highly efficient binding of His-tagged proteins and leads to high recovery rates with minimized nickel bleeding into the eluate.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni NTA-Beads may be regenerated, making them very cost-effective.The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10  mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eNickel charged NTA-agarose beads for affinity chromatography under reducing conditions. The matrix of choice for applications under medium pressure, with high flow rate or with big sample-\/matrix volume.In ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni NTA-HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate. ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His\/Ni HPBeads are ideal for purifying large sample volumes.50 % bead suspension in 20 % ethanol.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRapid one-step purification of very pure His-tagged proteins from total lysates \u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh binding capacity for 6xHis-tagged protein (≥60 mg\/ml purified His-tagged protein)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eReliably elution and regeneration\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eFor batch mode, gravity flow, MPLC und FPLC\u003c\/b\u003e\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eRecommended for big matrix volumes\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eSuitable for\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eMPLC and FPLC, or if His-tag proteins shall be isolated either\u003cb\u003e in particularly short time or in big amounts from reducing solutions\u003c\/b\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eMay be autoclaved at 121 °C for 30 mins.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eInstructions for use, addition\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tBead suspension, binding capacity ≥60 mg\/ml packed matrix (His)\u003csub\u003e6\u003c\/sub\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-0805-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w1OTI2MzN8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmc1WkM5b1pUSXZPVEEwTURjMU9EZ3pNekU0TWk1d1pHWXxkNzk0OTQwMjFjMTc2MzQzZGU3NmM0ZTY0NmE3OWM0YjhjYmMxNDQxNmE0OGNlMzIxMjA0ZmEzOTRmYzBkNWM4\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"25 ml \/ 1 piece","offer_id":56112599204172,"sku":"0805.1","price":628.9,"currency_code":"EUR","in_stock":true},{"title":"25 ml \/ 6+ pieces","offer_id":56112599236940,"sku":"0805.1","price":597.46,"currency_code":"EUR","in_stock":true},{"title":"25 ml \/ 24+ pieces","offer_id":56112599269708,"sku":"0805.1","price":566.01,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 1 piece","offer_id":56112599302476,"sku":"0805.2","price":2096.25,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ pieces","offer_id":56112599335244,"sku":"0805.2","price":1991.44,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ pieces","offer_id":56112599368012,"sku":"0805.2","price":1886.63,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/0805-1000Wx1000H.jpg?v=1766399055","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/roti-garose-his-ni-nta-hpbeads-0805","provider":"Luminix Health","version":"1.0","type":"link"}