{"product_id":"roti-load-2-k930-k930","title":"ROTI®Load 2, non reducing, 4x conc., 10 ml, glass","description":"\u003cp\u003eROTI®Load 2, non reducing, 4x conc., Density (D) 1,158 g\/cm³, Storage temp. +15 to +25 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 1 (reducing)\u003c\/b\u003e as well as \u003cb\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 2 and 3 (non-redu\u003c\/b\u003e\u003cb\u003ecing)\u003c\/b\u003e represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI\u003csup\u003e®\u003c\/sup\u003eLoad are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eWell tested and proven sample buffer for protein gel electrophoresis that has been modified and further developed based on the original formulation acc. to Laemmli (\u003ci\u003eNature\u003c\/i\u003e 227, 1970). For depiction of protein complexes, secondary structures, and for native gels.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFormulation acc. to Laemmli (modified)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eStabilises peptide bands\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFor protein complexes and secondary structures\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFor native gels\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad protein gel loading buffers are usually used as 1x concentrated solution. ROTI\u003csup\u003e®\u003c\/sup\u003eLoad can either be added to the prepared sample or the precipitated proteins are dissolved directly into ROTI\u003csup\u003e®\u003c\/sup\u003eLoad. Optionally, heat the ROTI\u003csup\u003e®\u003c\/sup\u003eLoad mixed sample to 95 °C for 5 minutes to break hydrogen bonds and stretch the peptide chains.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 2 prevents degradation of proteins while heating during sample preparation, additionally keeping the sample pH value constant during gel electrophoresis. Being a phosphate-buffered ready-to-use solution, ROTI\u003csup\u003e®\u003c\/sup\u003eLoad 2 already contains all necessary denaturing reagents, bromophenol blue as dye and glycerol as density increasing reagent. ROTI\u003csup\u003e®\u003c\/sup\u003eLoad 2 may be supplemented with 20 µl\/ml DTT solution (1 M), in order to gain a loading buffer using DTT as reducing reagent rather than β-Mercaptoethanol.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-K930-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wxMzMxNjF8YXBwbGljYXRpb24vcGRmfGFEWm1MMmc1Tmk4NU1UYzROemd5TnpZd09Ua3dMMEpCWDBzNU16QmZSVTR1Y0dSbXwzZGE1ODYxYTU5MWM0ODNkZDc1YjgxMGNlMWU1NmIzYzdiNmYwMWJjYzhlMDQ0Mjk4YWZhMzBmNTg0ZjAzYjJj\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"10 ml \/ 1 piece","offer_id":56182119596364,"sku":"K930.1","price":21.0,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 6+ pieces","offer_id":56182119629132,"sku":"K930.1","price":19.95,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 24+ pieces","offer_id":56182119661900,"sku":"K930.1","price":18.9,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/K929-1000Wx1000H_f3964055-eade-4be4-b246-93c2162901f1.jpg?v=1766480163","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/roti-load-2-k930-k930","provider":"Luminix Health","version":"1.0","type":"link"}