{"product_id":"roti-lux-probe-qpcr-master-mix-ohne-rox-25kp-25kp","title":"ROTI®Lux Probe qPCR Master Mix, ohne ROX, ready to use, 2x conc., 1 ml, plastic","description":"\u003cp\u003eROTI®Lux Probe qPCR Master Mix, ohne ROX, ready to use, 2x conc., 1 ml, Density (D) ~1 g\/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLux Probe qPCR Master Mixes are suitable for all common types of probe-based assays, for probe-based genotyping using multiplexing and for the analysis of gene expression in quantitative real-time PCR.The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the high sensitivity, even the smallest amounts of cDNA (\u0026lt;1pg) can be detected.The Master Mixes are also characterised by a particularly high temperature stability, which enables pipetting at room temperature and which means that they remain stable even after several Freeze and Thaw cycles (\u0026gt;10x).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eProperties:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh temperature stability\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eExcellent sensitivity (\u0026lt;1 pg cDNA)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eUltra-fast qPCR in \u0026lt;20 min\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCompatible with all common probe systems (primer probes, hydrolysis probes such as TaqMan\u003csup\u003e®\u003c\/sup\u003e, hybridization probes)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFor all common qPCR cyclers - optionally with or without ROX\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eProbe-based qPCR analyses\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMultiplexing\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGene expression analyses\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGenotyping\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eMaster Mix without the reference dye ROX (6-Carboxy-X-rhodamine)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eSuitable for:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eExicycler™(Bioneer)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCFX Real-Time PCR Cycler (Biorad)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRealLine Cycler (Bioron)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eDtlite \u0026amp; Dtprime Cycler (DNA Technology)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMastercycler\u003csup\u003e®\u003c\/sup\u003e (Eppendorf)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eLightCycler\u003csup\u003e®\u003c\/sup\u003e (Roche)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eAriaMx Real-time PCR System (Agilent)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eQ-Cycler (Quantabio)\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eStability at room temperature:\u003c\/b\u003e The ROTI\u003csup\u003e®\u003c\/sup\u003eLux qPCR Mastermixes can be stored at room temperature (≤30 °C) for at least 14 days.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eSensitivity and dynamic range:\u003c\/b\u003e The ROTI\u003csup\u003e®\u003c\/sup\u003eLux Probe qPCR Master Mixes deliver consistent and reliable results, even at low input amounts such as 0.1 pg cDNA, or 1 template per reaction.\u003c\/div\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eStability by multiple freeze and thaw cycles:\u003c\/b\u003e The stability of the ROTI\u003csup\u003e®\u003c\/sup\u003eLux qPCR master mixes is guaranteed even after multiple freeze and thaw  cycles (≥10).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eRobust sensitivity: \u003c\/b\u003eThe ROTI\u003csup\u003e®\u003c\/sup\u003eLux Probe qPCR Master Mixes provide stable and quantifiable results even at high Cq levels.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\u003cdiv\u003eNot a medical device \/ Not an IVD product\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tProbe-based real-time PCR assays \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAmplicon ends\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t3'dA \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eBasic material\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tDNA (cDNA \/ gDNA) \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003ePolymerase\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tHotStart DNA Polymerase \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eInstructions for use, addition\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tThe master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl\u003csub\u003e2 \u003c\/sub\u003eand the reaction buffer. Primer and template must be added separately.1 ml Master Mix is sufficient for one 96-well plate. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eVersion\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tno ROX \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-25KP-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wzOTIxODZ8YXBwbGljYXRpb24vcGRmfGFEUTRMMmd5TkM4NU1UVXpNVEUzTmpnek56UXlMMEpCWHpJMVMxQmZSVTR1Y0dSbXwyNWYwN2Q3NDc3NTk3ZDdkZmM4NmRhZjUyMDkzYTk0ZjgzMzIxZGUwZjM4ZGEyODNkYTliMTcyODI1YzBjYTk2\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 ml \/ 1 piece","offer_id":56128418414924,"sku":"25KP.1","price":80.65,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 6+ pieces","offer_id":56128418447692,"sku":"25KP.1","price":80.65,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 24+ pieces","offer_id":56128418480460,"sku":"25KP.1","price":80.65,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 1 piece","offer_id":56128418513228,"sku":"25KP.2","price":376.25,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 6+ pieces","offer_id":56128418545996,"sku":"25KP.2","price":357.44,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 24+ pieces","offer_id":56128418578764,"sku":"25KP.2","price":338.63,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 1 piece","offer_id":56128418611532,"sku":"25KP.3","price":698.75,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 6+ pieces","offer_id":56128418644300,"sku":"25KP.3","price":663.81,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 24+ pieces","offer_id":56128418677068,"sku":"25KP.3","price":628.88,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1000Wx1000H-25KP.jpg?v=1766414120","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/roti-lux-probe-qpcr-master-mix-ohne-rox-25kp-25kp","provider":"Luminix Health","version":"1.0","type":"link"}