{"product_id":"roti-pol-hot-taqhy-1-x-40-ul-9346","title":"ROTI®Pol Hot-TaqHY (1 x 40 µl), 5 U\/µl, 40 µl, plastic","description":"\u003cp\u003eROTI®Pol Hot-TaqHY (1 x 40 µl), 5 U\/µl, Storage temp. -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFor PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria \u003ci\u003eThermus aquaticus\u003c\/i\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003ePol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI\u003csup\u003e®\u003c\/sup\u003ePol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eModified version of the recombinant hot start Taq DNA polymerase from the thermophilic bacterium \u003ci\u003eThermus aquaticus\u003c\/i\u003e in storage buffer, plus additional 10x concentrated PCR reaction buffer and 10x concentrated PCR reaction buffer with red gel loading dye. ROTI\u003csup\u003e®\u003c\/sup\u003ePol Hot-TaqHY is recommended for use in highly specific PCR applications with particularly high yields. This polymerase set ROTI\u003csup\u003e®\u003c\/sup\u003ePol Hot-TaqHY is superior for all sophisticated Taq-based cycling protocols, in which both, particularly specific amplification as well as very high yields shall be obtained, or if highly specific amplifications shall be performed using particularly short cycle times.Thus, ROTI\u003csup\u003e®\u003c\/sup\u003ePol Hot-TaqHY is the perfect choice  prior to cloning or sequencing processes, for cycle sequencing and for colony PCR, and it may also well be used in qPCR- and multiplex PCR applications. Combining the benefits of both techniques, the Hot-TaqHY DNA polymerase has been designed by increasing the PCR sequence specificity of a quick and high yield modified Taq polymerase by antibody mediated inhibition. In combination with our unique buffers, the Hot-TaqHY polymerase delivers highly specific PCR amplification of superior yield with a wide range of PCR templates. The blocked Taq polymerase is getting active only after the initial denaturation step, resulting in highly specific amplification of the target sequence without production of unwanted side products due to unspecific primer annealing. In parallel, the modification of the DNA polymerase results in significantly enhanced amplicon yields, with the benefit of shorter elongation times needed. ROTI\u003csup\u003e®\u003c\/sup\u003ePol Hot-TaqHY is able to amplify PCR products up to 3 kb with genomic DNA and up to at least 5 kb in size with Lambda DNA and is appropriate for use in the amplification of DNA from a wide range of even complex templates. The Hot-TaqHY DNA polymerase included in the set possesses a 5' → 3' polymerase- as well as a 5'-flap endonuclease activity, and generates a 3'dA (adenine)-overhang which may well be used for TA-cloning purposes.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure: \u003c\/b\u003eSensitivity assay using ROTI\u003csup\u003e®\u003c\/sup\u003ePol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U\/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.M: 100 bp-DNA Ladder extended. NTC: no template control.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFilled in colour coded tubes, the set contains the DNA polymerase and two 10x concentrated reaction buffers with 20 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, one of which has been specially designed for direct gel loading following the PCR reaction. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The use of the colourless PCR reaction buffer is adequate for all general PCR applications and is particularly recommended when direct fluorescence or absorbance readings are required.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eThe set contains\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eHot-TaqHY polymerase in storage buffer containing 50 % glycerol, PCR buffer (10x) with 20 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, PCR buffer red (10x) with 20 mM MgCl\u003csub\u003e2\u003c\/sub\u003e and 0,1 % cresol red. \u003cb\u003eColour coded tubes.\u003c\/b\u003eContents of this set may not be bought separately.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\u003cdiv\u003eNot a medical device \/ Not an IVD product\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tParticularly sequence specific, fast PCR protocols and\/or high yield, GC rich template DNA \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAmplicon ends\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t3'dA \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003ePolymerase\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\u003ci\u003eHigh yield\u003c\/i\u003e \/ \u003ci\u003ehigh performance hot start\u003c\/i\u003e Taq polymerase \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eReaction buffer\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tcolourless + red (ready to load) \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eUse\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tParticularly sequence specific, fast PCR protocols and\/or high yield, GC rich template DNA \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-9346-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w1MzkyNTB8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmc0WkM5b01qQXZPVEExTnpjNU16ZzJOemd3Tmk1d1pHWXw4M2MzNjVmY2JmZDc1MGQzOGRiNDczN2Y5MWU4YWMxYzA0NGU4ZTY4OTkyYTMyYTBjMGQ3MjI3YjE4YWRjMjli\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"40 µl \/ 1 piece","offer_id":56427477074252,"sku":"9346.1","price":94.1,"currency_code":"EUR","in_stock":true},{"title":"40 µl \/ 6+ pieces","offer_id":56427477107020,"sku":"9346.1","price":89.4,"currency_code":"EUR","in_stock":true},{"title":"40 µl \/ 24+ pieces","offer_id":56427477139788,"sku":"9346.1","price":84.69,"currency_code":"EUR","in_stock":true},{"title":"200 µl \/ 1 piece","offer_id":56427477172556,"sku":"9346.2","price":424.65,"currency_code":"EUR","in_stock":true},{"title":"200 µl \/ 6+ pieces","offer_id":56427477205324,"sku":"9346.2","price":403.42,"currency_code":"EUR","in_stock":true},{"title":"200 µl \/ 24+ pieces","offer_id":56427477238092,"sku":"9346.2","price":382.19,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/9346-1000Wx1000H.jpg?v=1767622031","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/roti-pol-hot-taqhy-1-x-40-ul-9346","provider":"Luminix Health","version":"1.0","type":"link"}