{"product_id":"roti-sep-1077-human-sterile-0642","title":"ROTI®Sep 1077 human, sterile, for density gradient centrifugation, 100 ml","description":"\u003cp\u003eROTI®Sep 1077 human, sterile, for density gradient centrifugation, ready-to-use, CELLPURE®, Molar mass (M) ~400000 g\/mol, Density (D) 1,077 g\/cm³, Boiling point (bp) 100 °C, Storage temp. +4 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\u003cdiv\u003e\u003cb\u003eSterile\u003c\/b\u003e\u003c\/div\u003e\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eReady-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.Density 1.077 g\/ml.Best alternative to Ficoll\u003csup\u003e®\u003c\/sup\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOptimised for isolation of lymphocytes from non-coagulated human whole blood\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eProduces distinct, compact layers\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eResults in high recovery rates of viable cells\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMaintains the representative ratio of B- to T lymphocytes\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMay be directly applied to all protocols using Ficoll\u003csup\u003e®\u003c\/sup\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eSep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll\u003csup\u003e®\u003c\/sup\u003e this reagent may be replaced by ROTI\u003csup\u003e®\u003c\/sup\u003eSep 1077 human without any adjustment.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003eThe \u003cb\u003efigure\u003c\/b\u003e depicts two specimens prepared with heparinic blood obtained from venous puncture. Density gradient centrifugation of 6 ml diluted whole blood (dilution in PBS 1:1.5), with 3 ml of ROTI\u003csup\u003e®\u003c\/sup\u003eSep 1077 human, 10 mins., 1000 g, no break.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\u003cdiv\u003eNot a medical device \/ Not an IVD product\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-0642-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w1MTU2MzR8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmc0TkM5b1lXVXZPVEF6Tnpjek9ETXhNVGN4TUM1d1pHWXw5ZWJjZGU4OTdhMmJlYWIzODc2OWM1OTQ1ZTdiMjUxNTc1MDEzYmNjNjBlNmNhODNmMWU2NWVkZjlkZWYxYjY5\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"100 ml \/ 1 piece","offer_id":56112570335564,"sku":"0642.1","price":60.1,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ pieces","offer_id":56112570368332,"sku":"0642.1","price":57.1,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ pieces","offer_id":56112570401100,"sku":"0642.1","price":54.09,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 1 piece","offer_id":56112570433868,"sku":"0642.2","price":149.45,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 6+ pieces","offer_id":56112570466636,"sku":"0642.2","price":141.98,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 24+ pieces","offer_id":56112570499404,"sku":"0642.2","price":134.51,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/0642-1000Wx1000H.jpg?v=1766398991","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/roti-sep-1077-human-sterile-0642","provider":"Luminix Health","version":"1.0","type":"link"}