{"product_id":"rotitest-vital-0069","title":"ROTITEST® Vital, sterile, ready-to-use, 1 ml, plastic","description":"\u003cp\u003eROTITEST® Vital, sterile, ready-to-use, ROTItest® Bio Analysis, Density (D) 1,37 g\/cm³, Boiling point (bp) 100 °C, Storage temp. +4 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\u003cdiv\u003e\u003cb\u003eSterile\u003c\/b\u003e\u003c\/div\u003e\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eRapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eOptimum replacement for MTT:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCells keep on being vital\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMost simple handling\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOne-component-system without radioactivity\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFor adherent and suspension cells\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003ePhotometric result in 1 to 4 hours\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eStrong correlation between absorbance and cell number\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuitable for every culture media\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eSensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [\u003csup\u003e3\u003c\/sup\u003eH]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga \u003ci\u003eet al.\u003c\/i\u003e, \u003ci\u003eAnal. Commun.\u003c\/i\u003e 1999 (36) 47-50).In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST\u003csup\u003e®\u003c\/sup\u003evital is based on the (colourless) WST-8, which functions as acceptor for the NADH\/NADPH dehydrogenase while being reduced to (coloured) formazane during the process.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm).Also suitable for culture media containing phenol red.Each package contains simple-to-follow instructions-for-use.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFig.:\u003c\/b\u003e Example of use\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFig. A:\u003c\/b\u003e Correlation of cell counting results achieved with ROTITEST\u003csup\u003e®\u003c\/sup\u003e Vital (10 μl\/well) and \u003csup\u003e3\u003c\/sup\u003eH-Thymidine (37 KBq\/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM).\u003c\/div\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFig. B:\u003c\/b\u003e Comparison of cell counting results using ROTITEST\u003csup\u003e®\u003c\/sup\u003e Vital (10 μl\/well) and other cell counting reagents. Photometrical measurement at 450 nm (ROTITEST\u003csup\u003e®\u003c\/sup\u003e Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM).\u003c\/div\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFig. C:\u003c\/b\u003e Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with ROTITEST\u003csup\u003e®\u003c\/sup\u003e Vital (10 μl\/well).\u003cb\u003eFig. D:\u003c\/b\u003e Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST\u003csup\u003e®\u003c\/sup\u003e Vital (10 μl\/well).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-PapersOfRotitestVital-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3w1OTc1MzZ8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyZzFPUzlvTnpZdk9URXhOamN6T0RjeE5UWTNPQzV3WkdZfDQxOWUxZmExMjMzNzQzMjU3OWIzYWZjYmRiZGJlZWNlMjE3YzhkMWM1ZjBkODQwNTdmYWI1MTA0M2NlZjgxY2U\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-PapersOfRotitestVital-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3w1OTc1MzZ8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyZzFPUzlvTnpZdk9URXhOamN6T0RjeE5UWTNPQzV3WkdZfDQxOWUxZmExMjMzNzQzMjU3OWIzYWZjYmRiZGJlZWNlMjE3YzhkMWM1ZjBkODQwNTdmYWI1MTA0M2NlZjgxY2U\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    CE Declaration of Conformity (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 ml \/ 1 piece","offer_id":56112494641484,"sku":"0069.3","price":87.65,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 6+ pieces","offer_id":56112494674252,"sku":"0069.3","price":87.65,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 24+ pieces","offer_id":56112494707020,"sku":"0069.3","price":87.65,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 1 piece","offer_id":56112494739788,"sku":"0069.1","price":233.85,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 6+ pieces","offer_id":56112494772556,"sku":"0069.1","price":222.16,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 24+ pieces","offer_id":56112494805324,"sku":"0069.1","price":210.47,"currency_code":"EUR","in_stock":true},{"title":"20 ml \/ 1 piece","offer_id":56112494838092,"sku":"0069.2","price":704.15,"currency_code":"EUR","in_stock":true},{"title":"20 ml \/ 6+ pieces","offer_id":56112494870860,"sku":"0069.2","price":668.94,"currency_code":"EUR","in_stock":true},{"title":"20 ml \/ 24+ pieces","offer_id":56112494903628,"sku":"0069.2","price":633.74,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/0069-1000Wx1000H.jpg?v=1766398784","url":"https:\/\/www.luminixhealth.com\/de-de\/products\/rotitest-vital-0069","provider":"Luminix Health","version":"1.0","type":"link"}