{"product_id":"ligase-tth-3736","title":"Ligase Tth, 5 U\/µl, for Molecular Biology, 50 µl, plastic","description":"\u003cp\u003eLigase Tth, 5 U\/µl, for Molecular Biology, Density (D) 1,18 g\/cm³, Storage temp. -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eTth DNA Ligase catalyzes the NAD-dependent formation of phosphodiester bonds between adjacent 3'-hydroxyl and 5'-phosphate termini in double-stranded DNA. This ligase is inactive against single-stranded DNA or RNA and blunting DNA.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003estable at high temperatures\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eligates double-stranded DNA\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRepairs single strand breaks in double stranded DNA\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe Tth DNA ligase is stable and active at much higher temperatures than conventional DNA ligases. The optimal ligation temperature range is 7-10 °C higher than that of the T4 DNA ligase and is determined by the Tm of the substrates. High ligation temperature eliminates the nonspecific ligation.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eThe kit contains:\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eQuick LigaseLigation buffer (10x)The individual components of this kit cannot be purchased separately.The tubes included in the kit are colour coded to avoid confusion of reagents.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-3736-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w0OTc3Mzl8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmhpTXk5b1pqQXZPVEF6Tnpjek5EQXhPVEV3TWk1d1pHWXwxZmQ0NjU2MGY5ZTkxMzJiYzI1NzYzZmUyMmMwYWU1YjZhYjVlNmQ2NjAzOTZhZjM2NTYzMDM5ZDA4MDM5N2Y2\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"50 µl \/ 1 piece","offer_id":56150468067660,"sku":"3736.1","price":123.65,"currency_code":"EUR","in_stock":true},{"title":"50 µl \/ 6+ pieces","offer_id":56150468100428,"sku":"3736.1","price":117.47,"currency_code":"EUR","in_stock":true},{"title":"50 µl \/ 24+ pieces","offer_id":56150468133196,"sku":"3736.1","price":111.29,"currency_code":"EUR","in_stock":true},{"title":"500 µl \/ 1 piece","offer_id":56150468165964,"sku":"3736.2","price":794.45,"currency_code":"EUR","in_stock":true},{"title":"500 µl \/ 6+ pieces","offer_id":56150468198732,"sku":"3736.2","price":754.73,"currency_code":"EUR","in_stock":true},{"title":"500 µl \/ 24+ pieces","offer_id":56150468231500,"sku":"3736.2","price":715.01,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/3736-1000Wx1000H_3d095310-f7ca-498d-b127-fe17f2f16e75.jpg?v=1766435566","url":"https:\/\/www.luminixhealth.com\/en-be\/products\/ligase-tth-3736","provider":"Luminix Health","version":"1.0","type":"link"}