{"product_id":"zymolyase-100t-min-100-u-mg-9329","title":"Zymolyase® 100T, min. 100 U\/mg, for biochemistry and molecular biology, 100 mg","description":"\u003cp\u003eZymolyase® 100T, min. 100 U\/mg, for biochemistry and molecular biology, Storage temp. +4 °C, CAS No. 37340-57-1\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eZymolyase\u003csup\u003e®\u003c\/sup\u003e, produced by a submerged culture of \u003ci\u003eOerskovia xanthineolytica, \u003c\/i\u003ehas strong lytic activity against living cell walls of various strains of yeast cells. Hence, it is frequently used in order to produce protoplasts or spheroplasts. The essential enzyme for the lytic activity of Zymolyase\u003csup\u003e\u003csup\u003e®\u003c\/sup\u003e \u003c\/sup\u003eis β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eOther confirmed side activities\u003c\/b\u003e\u003cb\u003e: \u003c\/b\u003eβ-1,3-glucanase, protease, mannanase; occasionally traces of other hydrolytic activities (e.g. amylase, phosphatase).\u003cb\u003eStability: \u003c\/b\u003eAt 30 °C 70 % of the lytic activity is lost after 3 months. \u003cb\u003eOptimum temperature and pH:\u003c\/b\u003e at pH 7,5 - 35 °C for lysis of viable yeast cells, at pH 6,5 - 45 °C for hydrolysis of yeast glucan. \u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eUnit definition\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eOne unit of lytic activity is defined as the enzyme amount causing a decrease of 30 % in absorbance at 800 nm using 6 mg Brewer’s yeast as substrate in Phosphate buffer (pH 7,5) at 25 °C. Lytic activity varies depending on the particular yeast strain, the growth stage of the yeast, and cultural conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eZymolyase\u003csup\u003e®\u003c\/sup\u003e 100T is prepared by ammonium sulphate precipitatation, and is further purified by affinity chromatography.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAdditional info\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tSource: \u003ci\u003eArthro\u003c\/i\u003e\u003ci\u003ebacter luteus\u003c\/i\u003e \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/SPEZ-9329-EN.pdf?context=bWFzdGVyfHNwZWNpZmljYXRpb25zfDEwNDYyMHxhcHBsaWNhdGlvbi9wZGZ8YUdRMUwyZzBPQzg1TWpVME9UVTFNRFUzTVRneUwxTlFSVnBmT1RNeU9WOUZUaTV3WkdZfGFkZjU5MTUyZTczYjU2NzBjMzQxNjJjNWY4MWY2MzVkNDQxNjVhMzQwOTE2OTNkMjQ4OGUzYmU1YjZjYTEwOGE\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"100 mg \/ 1 piece","offer_id":56173508854092,"sku":"9329.1","price":375.2,"currency_code":"EUR","in_stock":true},{"title":"100 mg \/ 6+ pieces","offer_id":56173508886860,"sku":"9329.1","price":356.44,"currency_code":"EUR","in_stock":true},{"title":"100 mg \/ 24+ pieces","offer_id":56173508919628,"sku":"9329.1","price":337.68,"currency_code":"EUR","in_stock":true},{"title":"500 mg \/ 1 piece","offer_id":56173508952396,"sku":"9329.2","price":1192.2,"currency_code":"EUR","in_stock":true},{"title":"500 mg \/ 6+ pieces","offer_id":56173508985164,"sku":"9329.2","price":1132.59,"currency_code":"EUR","in_stock":true},{"title":"500 mg \/ 24+ pieces","offer_id":56173509017932,"sku":"9329.2","price":1072.98,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/9329-1000Wx1000H_ed8d83da-2bff-47be-b60d-5525459ad4e0.jpg?v=1766443930","url":"https:\/\/www.luminixhealth.com\/en-be\/products\/zymolyase-100t-min-100-u-mg-9329","provider":"Luminix Health","version":"1.0","type":"link"}