{"product_id":"plasmid-dna-pbr-sub-322-sub-x912-x912","title":"Plasmid DNA pBR322, lyophilized, 50 µg, plastic","description":"\u003cp\u003ePlasmid DNA pBR\u003csub\u003e322\u003c\/sub\u003e, lyophilized, Storage temp. -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form \u0026gt;95%) and, as a high-purity DNA (OD\u003csub\u003e260\/280\u003c\/sub\u003e \u0026gt; 1,70), it is ideal for different applications:\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eControlling transformation efficiency\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eComparison with plasmid ccc-forms of unknown size\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCloning DNA-fragments\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRestriction (Preparing DNA-length markers)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCalibrating DNA-separating or DNA-preparing processes or equipment\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eBlocking DNA-chips with plasmid DNA of a defined sequence\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe circular sequence was numbered in such a way that 0 lies in the middle of the singular \u003ci\u003eEcoR \u003c\/i\u003el restriction site. The copy number of pBR322 is limited by the gene product of \u003ci\u003erop\u003c\/i\u003e to 20 copies per cell. However, by adding chloroamphenicol (final concentration 170 µg\/ml) to log-phase cultures and then incubating for 8 hours, the copy number can be increased considerably.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eGeneral properties:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOD\u003csub\u003e260\/280\u003c\/sub\u003e: \u0026gt;1,70\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGenomic DNA: \u0026lt;2 %\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eoc form: \u0026lt;3 \u003cb\u003e%\u003c\/b\u003e\n\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eccc form: \u0026gt;95 %\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eBase pairs: 4361\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-X912-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wzMTU5MTh8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmcwT0M5b1l6SXZPVEF6TnpjeU5EazBNak0yTmk1d1pHWXwzMzIxYTg3MGYxMGNkODlkNzJlYjZkZTk5ZDM5MWE4MTA3Yzc5MjNhMzdlNjUwN2IwNmM2ZTdmNzEwYjg1MDg4\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"50 µg \/ 1 piece","offer_id":56186643677516,"sku":"X912.1","price":82.7,"currency_code":"EUR","in_stock":true},{"title":"50 µg \/ 6+ pieces","offer_id":56186643710284,"sku":"X912.1","price":78.57,"currency_code":"EUR","in_stock":true},{"title":"50 µg \/ 24+ pieces","offer_id":56186643743052,"sku":"X912.1","price":74.43,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/X912-1000Wx1000H.jpg?v=1766500582","url":"https:\/\/www.luminixhealth.com\/en-de\/products\/plasmid-dna-pbr-sub-322-sub-x912-x912","provider":"Luminix Health","version":"1.0","type":"link"}