{"product_id":"roti-fect-rnai-kit-3129","title":"ROTI®Fect RNAi Kit, ready-to-use, CELLPURE®, 0.2 ml, plastic","description":"\u003cp\u003eROTI®Fect RNAi Kit, ready-to-use, CELLPURE®, for transfection, Storage temp. +4 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eNew, powerful transfection reagent for liposoma mediated siRNA and miRNA transfection of all mammalian cells in culture. Liposoma formulation and enclosed buffer were specially established and designed for core parameters of RNAi: Most efficient quantitative endocytosis plus release of nucleic acids into the cytosol, without subsequent transport to the nucleus.ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect RNAi provides you with superior knockdown results, even using low amounts of RNA.Hereby, the ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect technology guarantees minimisation of toxic effects during transfection, thus making these reagents suitable for very sensitive cell lines of primary cells.The detailed instruction-for-use contains a standardised transfection protocol working via a definite RNA\/liposoma ratio and providing good results starting with the very first assay - making elaborate optimisations of transfection obsolete. Evaluation of results is often possible after 48 hours, enabling two sequential assays per week.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOptimised RNA release into the cytosol\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh levels of gene silencing with low RNA concentration\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eEfficient knockdown after 48 hours already\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eNo elaborate optimisation necessary\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVery low cell toxicity\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuitable for cell lines and primary cells\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eStraightforward, efficient protocol for whole medium included\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eSuccessfully tested on a variety of cell types.\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003ePrimary cells:\u003c\/b\u003e MCS, M1\u003cb\u003eCell lines:\u003c\/b\u003e A549, 3T3-L1, B16F10, CHO-K1, H441, HT1080, HeLa, HeLa-KB, HepG2\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eThe kit contains:\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e0.2 and 1 ml, respectively, ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect RNAi liposoma formulation (Art. No. 2911), and 0.4 and 2 ml, respectively, ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect RNAi buffer (Art. No. 2913). Les composants unitaires de ce kit ne peuvent pas être achetés séparément.Approx. 650 assays per ml in 24wells. Dependend on the cell line used.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure:\u003c\/b\u003e\u003ci\u003eKnockdown\u003c\/i\u003e of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10\u003csup\u003e4\u003c\/sup\u003e cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Measurement 2 days after transfection.\u003c\/div\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure:\u003c\/b\u003e\u003ci\u003eKnockdown\u003c\/i\u003e of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10\u003csup\u003e4\u003c\/sup\u003e cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Comparison of results obtained using different transfection reagents. Measurement 2 days after transfection.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-TransfectionProtocols-3129-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3wxMjI3OTl8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyZzNNaTlvTURBdk9URXhOamN6T0RneE16azRNaTV3WkdZfDY5MTA0ZGQ0YjQ2NDMzODFiODgyMzBiMWEyZmZlMWM2MTkxYjQ0OGI2ZjhkZGQ3MjczMTBmNWZiMzI0M2ZjOWQ\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"0.2 ml \/ 1 piece","offer_id":56148758364492,"sku":"3129.1","price":149.45,"currency_code":"EUR","in_stock":true},{"title":"0.2 ml \/ 6+ pieces","offer_id":56148758397260,"sku":"3129.1","price":141.98,"currency_code":"EUR","in_stock":true},{"title":"0.2 ml \/ 24+ pieces","offer_id":56148758430028,"sku":"3129.1","price":134.51,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 1 piece","offer_id":56148758462796,"sku":"3129.2","price":482.7,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 6+ pieces","offer_id":56148758495564,"sku":"3129.2","price":482.7,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 24+ pieces","offer_id":56148758528332,"sku":"3129.2","price":482.7,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1000Wx1000H-3129.jpg?v=1766432860","url":"https:\/\/www.luminixhealth.com\/en-de\/products\/roti-fect-rnai-kit-3129","provider":"Luminix Health","version":"1.0","type":"link"}