{"product_id":"roti-load-1-k929-k929","title":"ROTI®Load 1, reducing, 4x conc., 10 ml, glass","description":"\u003cp\u003eROTI®Load 1, reducing, 4x conc., Density (D) 1,1 to 1,2 g\/cm³, Storage temp. +15 to +25 °C, UN-Nr. 2810\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 1 (reducing)\u003c\/b\u003e as well as \u003cb\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 2 and 3 (non-redu\u003c\/b\u003e\u003cb\u003ecing)\u003c\/b\u003e represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI\u003csup\u003e®\u003c\/sup\u003eLoad are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eWell tested and proven sample buffer for protein gel electrophoresis that has been modified and further developed based on the original formulation acc. to Laemmli (\u003ci\u003eNature\u003c\/i\u003e 227, 1970). Optimized for a distinct band display. Recommended for size estimation of proteins in gel.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFormulation acc. to Laemmli (modified)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eStabilises peptide bands\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003ePrevents protein degradation\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eImproves gel separation\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad protein gel loading buffers are usually used as 1x concentrated solution. ROTI\u003csup\u003e®\u003c\/sup\u003eLoad can either be added to the prepared sample or the precipitated proteins are dissolved directly into ROTI\u003csup\u003e®\u003c\/sup\u003eLoad. Heat the ROTI\u003csup\u003e®\u003c\/sup\u003eLoad mixed sample to 95 °C for 5 minutes to break hydrogen bonds and stretch the peptide chains. The β-Mercaptoethanol contained in ROTI\u003csup\u003e®\u003c\/sup\u003eLoad 1 opens disulfide bridges by reduction and leads to a complete molecular stretching, the basic requirement for a correct size estimation of proteins in the gel.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 1 prevents degradation of proteins while heating during sample preparation, additionally keeping the sample pH value constant during gel electrophoresis. Being a phosphate-buffered ready-to-use solution, ROTI\u003csup\u003e®\u003c\/sup\u003eLoad 1 already contains all necessary denaturing and reducing reagents, bromophenol blue as dye and glycerol as density increasing reagent.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-K929-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wyMTE3Nzl8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmcwT0M5b1lqTXZPVEF6TnpjeU9EVXhOREEzT0M1d1pHWXwwY2VkMjc4MjM4ZWJmMTQxOGZhOTVmYTY4MTQwYTdjYjdlMDJhODIwYTM4MDAwZDlkMTEzMzJkMDI0YjAxMDZk\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"10 ml \/ 1 piece","offer_id":56182118973772,"sku":"K929.1","price":24.2,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 6+ pieces","offer_id":56182119006540,"sku":"K929.1","price":22.99,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 24+ pieces","offer_id":56182119039308,"sku":"K929.1","price":21.78,"currency_code":"EUR","in_stock":true},{"title":"40 ml \/ 1 piece","offer_id":56182119072076,"sku":"K929.2","price":73.0,"currency_code":"EUR","in_stock":true},{"title":"40 ml \/ 6+ pieces","offer_id":56182119104844,"sku":"K929.2","price":69.35,"currency_code":"EUR","in_stock":true},{"title":"40 ml \/ 24+ pieces","offer_id":56182119137612,"sku":"K929.2","price":65.7,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 1 piece","offer_id":56182119170380,"sku":"K929.3","price":160.2,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ pieces","offer_id":56182119203148,"sku":"K929.3","price":152.19,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ pieces","offer_id":56182119235916,"sku":"K929.3","price":144.18,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/K929-1000Wx1000H.jpg?v=1766480162","url":"https:\/\/www.luminixhealth.com\/en-de\/products\/roti-load-1-k929-k929","provider":"Luminix Health","version":"1.0","type":"link"}