{"product_id":"roti-lux-sybr-green-qpcr-master-mix-25ky-25ky","title":"ROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml","description":"\u003cp\u003eROTI®Lux SYBR®Green qPCR Master Mix, Low ROX, ready to use, 2x conc., 1 ml, Density (D) ~1 g\/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLux SYBR\u003csup\u003e®\u003c\/sup\u003eGreen qPCR Master Mixes are suitable for all common types of quantitative gene expression analyses and genotyping using real-time PCR.The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the particularly high sensitivity, just one template per reaction is sufficient for the analysis.The Master Mixes are also characterised by high temperature stability, which enables pipetting at room temperature and means that they remain stable even after several reeze and Thawcycles (\u0026gt;10x).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eProperties:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh temperature stability\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eExcellent sensitivity (\u0026lt;0.1 pg cDNA)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eUltra-fast qPCR in \u0026lt;20 min\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuitable for melting curve analysis (HRM)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFor all common qPCR cyclers - optionally with or without ROX\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGene expression analyses\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMelting curve analyses (HRM)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGenotyping\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eConcentration determination of NGS libraries\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eDetermination of copy number variants\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eTelomere length measurement\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003ePathogen detection\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eQuality control\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eMaster mix with low concentration (100 nM) of the reference dye ROX (6-Carboxy-X-rhodamine)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv class=\"\"\u003e\u003cstrong\u003eSuitable for:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eQuantstudio™ Cycler ( Thermofisher)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eqTower (Analytik Jena)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMx3000P\u003csup\u003e®\u003c\/sup\u003e qPCR Systeme (Agilent)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRotor-Gene™ (Qiagen)\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eStability by multiple freeze and thaw cycles:\u003c\/b\u003e The stability of the ROTI\u003csup\u003e®\u003c\/sup\u003eLux qPCR master mixes is guaranteed even after multiple freeze and thaw  cycles (≥10).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eSensitivity and dynamic range: \u003c\/b\u003eThe ROTI\u003csup\u003e®\u003c\/sup\u003eLux SYBR\u003csup\u003e®\u003c\/sup\u003eGreen qPCR Master Mixes deliver consistent and reliable results, even at low input amounts such as 0.1 pg cDNA, or 1 template per reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eStability at room temperature:\u003c\/b\u003e The ROTI\u003csup\u003e®\u003c\/sup\u003eLux qPCR Mastermixes can be stored at room temperature (≤30 °C) for at least 14 days.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\u003cdiv\u003eNot a medical device \/ Not an IVD product\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tGene expression analyses, SNP analyses, telomere length measurement \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eBasic material\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tDNA (cDNA \/ gDNA) \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003ePolymerase\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tHotStart DNA Polymerase \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eInstructions for use, addition\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tThe master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl\u003csub\u003e2 \u003c\/sub\u003eand the reaction buffer with the SYBR\u003csup\u003e®\u003c\/sup\u003eGreen dye. Primer and template must be added separately.1 ml Master Mix is sufficient for one 96-well plate. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eVersion\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tlow ROX \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-25KY-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wzOTE1OTl8YXBwbGljYXRpb24vcGRmfGFHWmhMMmhqTXk4NU1UWTNNelUwTkRZeU1qTTRMMEpCWHpJMVMxbGZSVTR1Y0dSbXxmMThjMTRmMTQ3ZWU1MTZkNDM2ZDgyMGIwYzlhZDRkMTAxZWJiZDQ0MTgxM2RlMDQ0NGI3YmNlYmZhNzY5YzE2\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 ml \/ 1 piece","offer_id":56128419004748,"sku":"25KY.1","price":80.65,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 6+ pieces","offer_id":56128419037516,"sku":"25KY.1","price":80.65,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 24+ pieces","offer_id":56128419070284,"sku":"25KY.1","price":80.65,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 1 piece","offer_id":56128419103052,"sku":"25KY.2","price":376.25,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 6+ pieces","offer_id":56128419135820,"sku":"25KY.2","price":357.44,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 24+ pieces","offer_id":56128419168588,"sku":"25KY.2","price":338.63,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 1 piece","offer_id":56128419201356,"sku":"25KY.3","price":698.75,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 6+ pieces","offer_id":56128419234124,"sku":"25KY.3","price":663.81,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 24+ pieces","offer_id":56128419266892,"sku":"25KY.3","price":628.88,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1000Wx1000H-25KY_0cd5a4b8-279c-4513-a9e3-fbd4ed0e9879.jpg?v=1766414120","url":"https:\/\/www.luminixhealth.com\/en-de\/products\/roti-lux-sybr-green-qpcr-master-mix-25ky-25ky","provider":"Luminix Health","version":"1.0","type":"link"}