{"product_id":"roti-phenol-chloroform-isoamyl-alcohol-a156-a156","title":"ROTI®Phenol\/Chloroform\/Isoamyl alcohol, for extraction of nucleic acids, 100 ml","description":"\u003cp\u003eROTI®Phenol\/Chloroform\/Isoamyl alcohol, for extraction of nucleic acids, ready-to-use, Density (D) 1,2 to 1,3 g\/cm³, Flash point (flp) \u0026gt;80 °C, Boiling point (bp) \u0026gt;61 °C, Storage temp. +4 °C, UN-Nr. 2810\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003ePlease note our \u003cb\u003eTechnical Information Brochure\u003c\/b\u003e on the Internet next to the products:\u003cb\u003ePhenolic DNA Isolation\u003c\/b\u003eBackground and protocol\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003ePhenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.For distinct phase separation, ROTI\u003csup\u003e®\u003c\/sup\u003eAqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003c\/p\u003e\n\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eReduce exposure to toxic chemicals\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003ePrepared from phenol of highest purity\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003ePacked under argon for maximum stability\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuccessfully tried and tested in many research laboratories\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003ePhenol\/Chloroform\/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol.ROTI\u003csup\u003e®\u003c\/sup\u003ePhenol\/Chloroform\/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI\u003csup\u003e®\u003c\/sup\u003ePhenol.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eAddition of 1 volume ROTI\u003csup\u003e®\u003c\/sup\u003ePhenol\/Chloroform\/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.Draw solution from lower phase. Do not shake before use!\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-PhenolicPurification-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3w0MDM0MTJ8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyZzVaUzlvWmpVdk9URXhOamN6T0RnME5qYzFNQzV3WkdZfDA5OTJhODEzM2ZlMWFiYzE4MDc0YjFhMGZhZGVlNzQyMDc4MmJmMTExOTQ3ZDI0OTdlZmI5Y2ZhNzFhZjMxYjQ\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"100 ml \/ 1 piece","offer_id":56173604897100,"sku":"A156.3","price":48.95,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ pieces","offer_id":56173604929868,"sku":"A156.3","price":46.5,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ pieces","offer_id":56173604962636,"sku":"A156.3","price":44.06,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 1 piece","offer_id":56173604995404,"sku":"A156.1","price":96.65,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 6+ pieces","offer_id":56173605028172,"sku":"A156.1","price":91.82,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 24+ pieces","offer_id":56173605060940,"sku":"A156.1","price":86.99,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 1 piece","offer_id":56173605093708,"sku":"A156.2","price":166.65,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 6+ pieces","offer_id":56173605126476,"sku":"A156.2","price":158.32,"currency_code":"EUR","in_stock":true},{"title":"500 ml \/ 24+ pieces","offer_id":56173605159244,"sku":"A156.2","price":149.99,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/A156-1000Wx1000H_207a510c-6d78-4cda-87af-251152b6c123.jpg?v=1766444205","url":"https:\/\/www.luminixhealth.com\/en-de\/products\/roti-phenol-chloroform-isoamyl-alcohol-a156-a156","provider":"Luminix Health","version":"1.0","type":"link"}