{"product_id":"dab-metal-enhancer-9204","title":"DAB Metal Enhancer, ready-to-use, for immunochemistry, 100 ml, plastic","description":"\u003cp\u003eDAB Metal Enhancer, ready-to-use, for immunochemistry, Density (D) ~1 g\/cm³, Boiling point (bp) 100 °C, Storage temp. +15 to +25 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eDAB metal enhancer is used to enhance the signal strength in peroxidase (HRP) detections and to modify the signal colour from brown to purplish gray. Applications include all immunochemical assays like immunohistochemistry and -cytochemistry as well as Western blotting, but DAB metal enhancer may also well be used during \u003ci\u003ein situ\u003c\/i\u003e hybridization.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eWhen the DAB metal enhancer is applied, the sensitivity of DAB staining is about two times higher than with DAB alone.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure: \u003c\/b\u003eImmunohistochemical staining of mouse small intestines with an anti-PCNA antibody. Minuscules: pure DAB substrate, capitals: detection supplemented by DAB enhancer (A: NiCl\u003csub\u003e2\u003c\/sub\u003e, B: DAB Metal Enhancer, Art. No. 9204).A\/a: Basic method using 0.6 mg\/ml DAB in Tris-buffer.B\/b: Detection performed with the ROTI\u003csup\u003e®\u003c\/sup\u003eDAB Kit.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFor application, the DAB substrate components and buffer reagents (for instance ROTI\u003csup\u003e®\u003c\/sup\u003eDAB-Kit, Art. No. 9202) are simply diluted in DAB metal enhancer instead of water. The purplish gray precipitate resulting from the staining reaction can be detected in visible light and does not bleach during long-term storage. Since it is insoluble in aqueous and organic solvents, slides may be permanently mounted in hydrophilic, or, after dehydration, in hydrophobic mounting medium.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure 1: \u003c\/b\u003eDot blot of a HRP conjugated antibody (400, 200, 100, 50 and 25 pg).CR\/cr: Detection using ROTI\u003csup\u003e®\u003c\/sup\u003eDAB KitA-e: Detection using kits and DAB substrates from other suppliersCR: Detection under supplementation with DAB Metal EnhancerA: Detection under supplementation with NiCl\u003csub\u003e2\u003c\/sub\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-9204-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wxODE2OTJ8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmczTVM5b1ltRXZPVEF6Tnpjek5EVXhNRFl5TWk1d1pHWXxlN2IzM2MwMDczMzBjZDNhMTAwMDU4MGU3MmU4NGI2NmIyZjgzZTM4ODA2M2YxNGVmMTgxNjZmNmEzZWI1MDc4\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"100 ml \/ 1 piece","offer_id":56173491716428,"sku":"9204.1","price":131.7,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ pieces","offer_id":56173491749196,"sku":"9204.1","price":125.12,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ pieces","offer_id":56173491781964,"sku":"9204.1","price":118.53,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/9204-1000Wx1000H_d43354f7-b293-4e66-9417-2a7ef4f1eaaa.jpg?v=1766443882","url":"https:\/\/www.luminixhealth.com\/en-fr\/products\/dab-metal-enhancer-9204","provider":"Luminix Health","version":"1.0","type":"link"}