{"product_id":"roti-fect-p001-p001","title":"ROTI®Fect, ready-to-use, CELLPURE®, 0.2 ml, plastic","description":"\u003cp\u003eROTI®Fect, ready-to-use, CELLPURE®, for transfection, Density (D) ~1 g\/cm³, Storage temp. +4 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eROTI\u003csup\u003e®\u003c\/sup\u003eFect \u003c\/b\u003eis a liposome-based transfection reagent consisting of a mixture of polycationic and neutral lipids. Compared to monocationic lipid formulations, ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently.An efficient triple-cotransfection using ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) \u003ci\u003eBiospektrum\u003c\/i\u003e 4: 464-5.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHighly efficient reproduction of DNA in mammal cells\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eExcellent reproducibility\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh and wide operating plateau\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eNo inhibition through serum\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eLow cell toxicity\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuccessful with different cell lines\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eExcellent transfection results\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eSuccessfully tested on a variety of cell types.\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003esiRNA:\u003c\/b\u003e CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620\u003cb\u003ePrimary cells:\u003c\/b\u003e pAML, pHDF, pMEF, pRHC, pSMC\u003cb\u003eCell lines:\u003c\/b\u003e 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, \u003ci\u003eet al.\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eApprox. 400 assays per ml in 6 wells. Dependent on the cell line used.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure:\u003c\/b\u003eTransfection of a variety of cell lines with ROTI\u003csup\u003e®\u003c\/sup\u003eFect and other commercially available transfectants.Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI\u003csup\u003e®\u003c\/sup\u003eFect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI\u003csup\u003e®\u003c\/sup\u003eFect was set to 100 %.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-TransfectionProtocols-P001-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3w1Mjk1MzB8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyZzRNaTlvTnpZdk9URXhOamN6T0RJMU5qa3lOaTV3WkdZfGI2MWZmNjMwOTJiOGZiNTkxY2Q5YWUyMTRiOGVkZjQ5M2YyMTQxNzAwNTZjMDA4OGY5YTFjZjEwYjEwNWMyZmY\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"0.2 ml \/ 1 piece","offer_id":56184710824268,"sku":"P001.1","price":103.75,"currency_code":"EUR","in_stock":true},{"title":"0.2 ml \/ 6+ pieces","offer_id":56184710857036,"sku":"P001.1","price":98.56,"currency_code":"EUR","in_stock":true},{"title":"0.2 ml \/ 24+ pieces","offer_id":56184710889804,"sku":"P001.1","price":93.38,"currency_code":"EUR","in_stock":true},{"title":"0.5 ml \/ 1 piece","offer_id":56184710922572,"sku":"P001.2","price":220.4,"currency_code":"EUR","in_stock":true},{"title":"0.5 ml \/ 6+ pieces","offer_id":56184710955340,"sku":"P001.2","price":209.38,"currency_code":"EUR","in_stock":true},{"title":"0.5 ml \/ 24+ pieces","offer_id":56184710988108,"sku":"P001.2","price":198.36,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 1 piece","offer_id":56184711020876,"sku":"P001.3","price":344.0,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 6+ pieces","offer_id":56184711053644,"sku":"P001.3","price":344.0,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 24+ pieces","offer_id":56184711086412,"sku":"P001.3","price":344.0,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 1 piece","offer_id":56184711119180,"sku":"P001.4","price":1531.9,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 6+ pieces","offer_id":56184711151948,"sku":"P001.4","price":1455.31,"currency_code":"EUR","in_stock":true},{"title":"5 ml \/ 24+ pieces","offer_id":56184711184716,"sku":"P001.4","price":1378.71,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1000Wx1000H-P001_f4d9dba0-6701-4545-95b3-75a577de3753.jpg?v=1766493541","url":"https:\/\/www.luminixhealth.com\/en-fr\/products\/roti-fect-p001-p001","provider":"Luminix Health","version":"1.0","type":"link"}