{"product_id":"roti-load-3-lds-3359","title":"ROTI®Load 3 (LDS), non reducing, 4x conc., 10 ml, glass","description":"\u003cp\u003eROTI®Load 3 (LDS), non reducing, 4x conc., Density (D) 1,134 g\/cm³, Storage temp. +15 to +25 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 1 (reducing)\u003c\/b\u003e as well as \u003cb\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad 2 and 3 (non-redu\u003c\/b\u003e\u003cb\u003ecing)\u003c\/b\u003e represent special gel loading buffers for protein gel electrophoresis. They have been designed to protect your proteins during sample preparation and to stabilise sensitive peptide bonds. Protein degradation is efficiently prevented by those buffer systems. ROTI\u003csup\u003e®\u003c\/sup\u003eLoad are 4x concentrated ready-to-use solutions, which may be used directly for sample loading.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eModified, non-reducing protein sample-gel loading buffer for gels under denaturing or native conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOptimised for ready-made gels and cooled gel runs\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuperior solubility at low temperatures\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eStabilises proteins in the gel\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLoad protein gel loading buffers are usually used as 1x concentrated solution. ROTI\u003csup\u003e®\u003c\/sup\u003eLoad can either be added to the prepared sample or the precipitated proteins are dissolved directly into ROTI\u003csup\u003e®\u003c\/sup\u003eLoad. Optionally, heat the ROTI\u003csup\u003e®\u003c\/sup\u003eLoad mixed sample to 95 °C for 5 minutes to break hydrogen bonds and stretch the peptide chains.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eDuring sample preparation and heating, ROTI\u003csup\u003e®\u003c\/sup\u003eLoad 3 (LDS) efficiently prevents degradation of your proteins, also stabilising the pH value during the run. This gel loading buffer is based on a triethanolamine buffered ready-to-use solution, in which the standard sodium dodecylsulphate (SDS) has been replaced by lithium dodecylsulphate (LDS). At low temperatures, LDS is much better soluble than SDS. Hence, no crystallisation of LDS takes place, and protein stability as well as band resolution is significantly enhanced. Additionally, the formulation of ROTI\u003csup\u003e®\u003c\/sup\u003eLoad 3 (LDS) has been optimised for ready-made gels, particularly Q-PAGE™ gels, but also ready-made gels of other standard suppliers.For reducing conditions, ROTI\u003csup\u003e®\u003c\/sup\u003eLoad 3 (LDS) may be supplemented with 20 µl\/ml DTT solution (1 M) or with β-mercaptoethanol.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-3359-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wyMDg4NjB8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmd3Tmk5b01URXZPVEF6Tnpjek5EQTFNVGczTUM1d1pHWXwzODE3M2VkZjk3MWEzMDFjNDE1MTU4NGZmZDA1MjhiMDk4NDAyNDFjZWZlNzg5NjFkYmRkNDE5NTFjOWMwNzdm\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"10 ml \/ 1 piece","offer_id":56149218787660,"sku":"3359.1","price":27.45,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 6+ pieces","offer_id":56149218820428,"sku":"3359.1","price":26.08,"currency_code":"EUR","in_stock":true},{"title":"10 ml \/ 24+ pieces","offer_id":56149218853196,"sku":"3359.1","price":24.71,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/K929-1000Wx1000H_40e537f3-497f-4b8e-a8b4-182711427ce5.jpg?v=1766434377","url":"https:\/\/www.luminixhealth.com\/en-fr\/products\/roti-load-3-lds-3359","provider":"Luminix Health","version":"1.0","type":"link"}