{"product_id":"roti-lumin-p078-p078","title":"ROTI®Lumin, 2 x 25 ml, 10x conc., fluorescence-free","description":"\u003cp\u003eROTI®Lumin, 10x conc., fluorecent-free, for protein and cytoimmunochemistry, Storage temp. +4 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSensitive and reliable\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFor all standard membrane types\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eLow background\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eNo cumbersome preparation of peroxidase buffers\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuitable for multiple stripping and hybridisation\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLumin is a kit for chemoluminescent detection of peroxidase-linked reporters (e.g. HRP-antibodies), as can be found in Western-Blot assays. In comparison to chromogen substrates, ROTI\u003csup\u003e®\u003c\/sup\u003eLumin displays increased sensitivity and reduced background reaction.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLumin is transformed by the HRP into an excited dianion in the presence of hydrogen peroxide. When the dianion is reversed back to its normal state, light is emitted. The light emission of ROTI\u003csup\u003e®\u003c\/sup\u003eLumin reaches its maximum within 5 minutes and remains at the maximum level for approx. 1-2 hours.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eLumin consists of two solutions, which should be mixed at a 1:1 ratio before use. It can be used with nitrocellulose, nylon and PVDF membranes. Optimal results can be achieved with Immobilon-P\u003csup\u003e®\u003c\/sup\u003e PVDF or ROTI\u003csup\u003e®\u003c\/sup\u003ePVDF. Can be used for Western-Blotting, ELISA, Southern- Blotting, Dot-Blotting and colony hybridisation. Membranes may be stripped from bound probes using ROTI\u003csup\u003e®\u003c\/sup\u003eFree stripping buffer, and may thus undergo multiple reprobing.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure: \u003c\/b\u003eSensitivity of the ROTI\u003csup\u003e®\u003c\/sup\u003eLumine.Sensitivity of ROTI\u003csup\u003e®\u003c\/sup\u003eLumin ultra is found in low femtogram range. Detection of a polyclonal antibody (left to right 4 pg, 400 fg, 40 fg, 4 fg) on ROTI\u003csup\u003e®\u003c\/sup\u003ePVDF (Art. No. T830.1). Staining in PBST via a HRP-conjugated anti-rabbit antibody (1:2000).a\/A: ROTI\u003csup\u003e®\u003c\/sup\u003eLumin ultra; b\/B: ROTI\u003csup\u003e®\u003c\/sup\u003eLumin plus; c\/C: ROTI\u003csup\u003e®\u003c\/sup\u003eLumin; d\/D: Product of a competitor, recommended for femtogram range. a\/b\/c\/d: 10 sec. exposition, A\/B\/C\/D: 1 min. exposition.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eThe kit contains\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e120 ml (25 ml) ROTI\u003csup\u003e®\u003c\/sup\u003eLumin 1 (Art. No. P079) and 120 ml (25 ml) ROTI\u003csup\u003e®\u003c\/sup\u003eLumin 2 (Art. No. P080).Contents of this Kit may not be bought separately.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tWestern, \u003ci\u003ein situ\u003c\/i\u003e, Histochemistry \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eEnzyme\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tHorseradish Peroxidase \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eDetection\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tchemoluminescent \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eSignal product\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tPrecipitate (Blot) \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eUse\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tWestern \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-RapidImmunodetection-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3wxNDUwNTR8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyZzRZeTlvTW1Rdk9URXhOalkwTWpRME16STVOQzV3WkdZfDViNGMwZDM2NzVkNzFkMjhkNzJhZTNhMjY2NWZkMTVhYzAxNzQyNDRiZGEyNTNlYjMxMTcyOWQ5NDcyYjA2MDQ\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 piece \/ 1 piece","offer_id":56184727208268,"sku":"P078.2","price":142.45,"currency_code":"EUR","in_stock":true},{"title":"6+ pieces \/ 6+ pieces","offer_id":56184727241036,"sku":"P078.2","price":135.33,"currency_code":"EUR","in_stock":true},{"title":"24+ pieces \/ 24+ pieces","offer_id":56184727273804,"sku":"P078.2","price":128.21,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/P078-1000Wx1000H_61a8b2c1-9d34-44f7-9486-e16ca1edc18f.jpg?v=1766493566","url":"https:\/\/www.luminixhealth.com\/en-fr\/products\/roti-lumin-p078-p078","provider":"Luminix Health","version":"1.0","type":"link"}