{"product_id":"roti-quick-kit-a979-a979","title":"ROTI®Quick kit, ready-to-use, for molecular biology, 1 kit, cardboard","description":"\u003cp\u003eROTI®Quick kit, ready-to-use, for molecular biology, Density (D) 0,786 g\/cm³, Storage temp. +4 °C, UN-Nr. 2924\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eColumn-free, ultra-flexible isolation system designed for reliable isolation of total RNA from almost any tissue.The ROTI\u003csup\u003e®\u003c\/sup\u003eQuick Kit is suitable for use with cell culture material, biopsies, high-fat or high-protein tissue, blood (Üçeyler \u003ci\u003eet al. Neurology\u003c\/i\u003e (2007) 69:42-9), solid plant material, mitochondria (Backert \u003ci\u003eet al. Plant Molecular Biology\u003c\/i\u003e (1997) 33:1037-50), mycoplasms (Weiner \u003ci\u003eet al. Nucleic Acids Res.\u003c\/i\u003e (2000) 28:4488-96) and many more. The kit can be used for both fresh tissue and frozen material.The ROTI\u003csup\u003e®\u003c\/sup\u003eQuick Kit is based on the GITC isolation method by Chomczynski and Sacchi (Chomczynski and Sacchi. (1987) \u003ci\u003eAnal. Biochem.\u003c\/i\u003e 162:156) and includes three optimised ready-to-use solutions that were developed by molecular biologists and have proven effective for many years.After the tissue has been homogenised, the RNA is purified in a single step and isolated by precipitation. Each isolation takes approximately 2,5 hours, and multiple isolations can easily be performed simultaneously. The incubation and centrifuging steps take about 2 hours.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eEasy, most versatile application\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh recovery rate of intact, very pure total RNA\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eColumn-free two-step isolation\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eApplicable on nearly any tissue type\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003ePreparation as given in the instruction-for-use is optimised for RNA isolation from approx. 0,2 g of tissue or cell material. The amount of material isolated per batch may easily be up- or downscaled to larger or smaller amounts of tissue.The isolated total RNA is highly pure and may be used directly for all common down-stream applications, such as RT-PCR, Northern blotting and reverse transcription. Detailed instructions-for-use are enclosed with each kit.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eThe kit contains:\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eQuick Kit 1, ROTI\u003csup\u003e®\u003c\/sup\u003eQuick Kit 2 and ROTI\u003csup\u003e®\u003c\/sup\u003eQuick Kit 3.Contents of this Kit may not be bought separately.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-A979-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w4MDIyNzF8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmc0Tnk5b1kyRXZPVEExTXpnM016ZzVOelV3TWk1d1pHWXxkMTRlMjNiMzlhYzNlNjNiMTM2ZTNkMTI2YmVmZDZjZjBlOTEyMjE1ZTZlMzAzNjFkNGZkZjUzYmYyNDk1YjBi\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 piece \/ 1 piece","offer_id":56174378156364,"sku":"A979.1","price":117.2,"currency_code":"EUR","in_stock":true},{"title":"6+ pieces \/ 6+ pieces","offer_id":56174378189132,"sku":"A979.1","price":111.34,"currency_code":"EUR","in_stock":true},{"title":"24+ pieces \/ 24+ pieces","offer_id":56174378221900,"sku":"A979.1","price":105.48,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/A979-1000Wx1000H_24e3c34a-58ca-44f3-8ffb-85de4baadf7c.jpg?v=1766444532","url":"https:\/\/www.luminixhealth.com\/en-fr\/products\/roti-quick-kit-a979-a979","provider":"Luminix Health","version":"1.0","type":"link"}