{"product_id":"vivacon-500-pcr-grade-100000-mw-100box-vivacon-500-pcr-grade-100000-mw-100p","title":"Vivacon 500-PCR Grade 100,000 MW 100\/BOX, Vivacon 500-PCR Grade, 100,000 MW 100p","description":"Vivacon® 500 is optimized for the concentration \/ recovery and buffer exchange applications of genomic DNA and peptides for samples up to 500 µl initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 500 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 500 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations. \u003csmall\u003e1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62.\u003c\/small\u003e Concentration of up to 0.5 ml genomic DNA or peptide samples Low binding material Reverse spin possibility MWCO printed on device Complete and highly reproducible concentrate recovery Selected cut offs available sterile and DNA free* for e.g. forensic applications * qPCR assay over 55 cycles with a verified limit of detection \u0026lt; 2 pg Performance Characteristics for DNA Start volume 0.5 ml, sample concentration 50 ng\/ml. \u003c!-- Table - Vivacon® 500 --\u003e \u003ctable\u003e \u003cthead\u003e \u003ctr\u003e \u003cth scope=\"\" col\u003e \u003c\/th\u003e \u003cth scope=\"\" col\u003e Sample size (bp)\u003c\/th\u003e \u003cth scope=\"\" col\u003e Time to concentrate up to 30x [min.] at 20°C\u003c\/th\u003e \u003cth scope=\"\" col\u003e Concentrate recovery %\u003c\/th\u003e \u003cth scope=\"\" col\u003e g-force (xg)\u003c\/th\u003e \u003c\/tr\u003e \u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003e 2,000 MWCO\u003c\/td\u003e \u003ctd\u003e 10\u003c\/td\u003e \u003ctd\u003e 60 min.\u003c\/td\u003e \u003ctd\u003e 93 %\u003c\/td\u003e \u003ctd\u003e 7,500\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 10,000 MWCO\u003c\/td\u003e \u003ctd\u003e 30\u003c\/td\u003e \u003ctd\u003e 25 min.\u003c\/td\u003e \u003ctd\u003e 94 %\u003c\/td\u003e \u003ctd\u003e 7,500\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 30,000 MWCO\u003c\/td\u003e \u003ctd\u003e 50\u003c\/td\u003e \u003ctd\u003e 18 min.\u003c\/td\u003e \u003ctd\u003e 88 %\u003c\/td\u003e \u003ctd\u003e 5,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 50,000 MWCO\u003c\/td\u003e \u003ctd\u003e 300\u003c\/td\u003e \u003ctd\u003e 18 min.\u003c\/td\u003e \u003ctd\u003e 91 %\u003c\/td\u003e \u003ctd\u003e 5,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 100,000 MWCO\u003c\/td\u003e \u003ctd\u003e 600\u003c\/td\u003e \u003ctd\u003e 10 min.\u003c\/td\u003e \u003ctd\u003e 87 %\u003c\/td\u003e \u003ctd\u003e 3,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 125,000 MWCO\u003c\/td\u003e \u003ctd\u003e 650\u003c\/td\u003e \u003ctd\u003e 12 min.\u003c\/td\u003e \u003ctd\u003e 85 %\u003c\/td\u003e \u003ctd\u003e 2,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 125,000 MWCO\u003c\/td\u003e \u003ctd\u003e 900\u003c\/td\u003e \u003ctd\u003e 9 min.\u003c\/td\u003e \u003ctd\u003e 94 %\u003c\/td\u003e \u003ctd\u003e 3,000\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e Performance Characteristics for Proteins Start volume 0.5 ml, sample and concentration of proteins as specified in table. \u003c!-- Table 2 - Vivacon® 500 --\u003e \u003ctable\u003e \u003cthead\u003e \u003ctr\u003e \u003cth scope=\"\" col\u003e \u003c\/th\u003e \u003cth scope=\"\" col\u003e Sample\u003c\/th\u003e \u003cth scope=\"\" col\u003e Time to concentrate up to 30x [min.] at 20°C\u003c\/th\u003e \u003cth scope=\"\" col\u003e Concentrate recovery %\u003c\/th\u003e \u003cth scope=\"\" col\u003e g-force (xg)\u003c\/th\u003e \u003c\/tr\u003e \u003c\/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd\u003e 2,000 MWCO\u003c\/td\u003e \u003ctd\u003e 0.25 mg\/ml cytochrome c\u003c\/td\u003e \u003ctd\u003e 30 min.\u003c\/td\u003e \u003ctd\u003e 95 %\u003c\/td\u003e \u003ctd\u003e 14,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 10,000 MWCO\u003c\/td\u003e \u003ctd\u003e 0.25 mg\/ml cytochrome c\u003c\/td\u003e \u003ctd\u003e 15 min.\u003c\/td\u003e \u003ctd\u003e 92 %\u003c\/td\u003e \u003ctd\u003e 14,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 30,000 MWCO\u003c\/td\u003e \u003ctd\u003e 1.0 mg\/ml BSA\u003c\/td\u003e \u003ctd\u003e 10 min.\u003c\/td\u003e \u003ctd\u003e 95 %\u003c\/td\u003e \u003ctd\u003e 14,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 50,000 MWCO\u003c\/td\u003e \u003ctd\u003e 1.0 mg\/ml BSA\u003c\/td\u003e \u003ctd\u003e 10 min.\u003c\/td\u003e \u003ctd\u003e 92 %\u003c\/td\u003e \u003ctd\u003e 14,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 100,000 MWCO\u003c\/td\u003e \u003ctd\u003e 1.0 mg\/ml bovine IgG\u003c\/td\u003e \u003ctd\u003e 11 min.\u003c\/td\u003e \u003ctd\u003e 90 %\u003c\/td\u003e \u003ctd\u003e 8,000\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003ctd\u003e 125,000 MWCO\u003c\/td\u003e \u003ctd\u003e 1.0 mg\/ml bovine IgG\u003c\/td\u003e \u003ctd\u003e 10 min.\u003c\/td\u003e \u003ctd\u003e 81 %\u003c\/td\u003e \u003ctd\u003e 8,000\u003c\/td\u003e \u003c\/tr\u003e \u003c\/tbody\u003e \u003c\/table\u003e","brand":"IDL","offers":[{"title":"Case of 100","offer_id":56334734393676,"sku":"VN01H42ETO","price":573.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/26-LT_Vivacon--_0402_RGB_62988855-aff7-474e-bf03-fe5413cbffdc.png?v=1766848995","url":"https:\/\/www.luminixhealth.com\/en-fr\/products\/vivacon-500-pcr-grade-100000-mw-100box-vivacon-500-pcr-grade-100000-mw-100p","provider":"Luminix Health","version":"1.0","type":"link"}