Description
MCDB 153 medium (modified) is a cell culture medium derived from Ham's nutrient mixture F-12 and intended for the growth of non-transformed cell types in a serum-free formulation. The 500 ml unit contains L-glutamine, phenol red, sodium bicarbonate and sodium pyruvate, together with hydrocortisone, T3, testosterone, insulin, transferrin, sodium selenite, MEM non-essential amino acids and glucose. This defined composition supports laboratory workflows that require a controlled nutrient base without serum. The medium is offered by manufacturer Sartorius as a practical option for research groups maintaining non-transformed cells under serum-free conditions. Each pack has a net weight of 0,590 kg and is suited to routine cell-culture applications in biomedical and life-science laboratories. By combining the classic MCDB 153 backbone with the listed supplements, the formulation supplies essential amino acids, energy sources, hormones and trace elements needed for defined culture systems. It integrates readily into existing serum-free protocols where a modified Ham's F-12-based medium is specified.
This article is part of Cell culture media & sera, in Cell culture.
Key features
- Pack size: 500 ml
- Storage temperature: 2–8 °C
- Medium type: MCDB 153 (modified)
- Base: Ham's nutrient mixture F-12
- Key components: L-glutamine, phenol red, sodium bicarbonate, sodium pyruvate
- Supplements: hydrocortisone, T3, testosterone, insulin, transferrin, sodium selenite
Recently viewed
You may also like
Browse related categories
Category
Sub-category
Catalogue
Brand
Questions about MODIFIED MCDB-153
What is this article?
MODIFIED MCDB-153 is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Cell culture media & sera, inside Cell culture, in Microbiology lab supplies.
Cell-culture medium is the liquid a cell line grows in. Serum, often fetal bovine, supplies what the basal medium does not. With or without glutamine, with or without phenol red, and the serum origin are different products. Serum is a cold-chain article and is never part of free standard delivery.
Cell culture grows cells outside the body. The medium, often with serum, supplies what the flask does not. A flask or a dish is the vessel, treated so the cells attach or left untreated so they float. Contamination by microbes, or by another cell line, is the ordinary failure. Cold-chain articles in this work, including serum, are never part of free standard delivery. Lines are catalogued by ATCC. Papers are on PubMed. Practice is summarised by the WHO laboratory biosafety topic.
Which category should I open next?
The category is Cell culture. The sub-category is Cell culture media & sera. The catalogue is Microbiology lab supplies.
Shelves beside this one are Cell culture accessories, Cell culture flasks, Cell culture plates & dishes and Cell strainers, inserts & scrapers.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.