Description
ROTI®ProBind PVDF transfer membrane is a polyvinylidene fluoride blotting membrane with a pore size of 0,45 µm, supplied as pre-cut sheets measuring 10 × 10 cm. It is suited to transfer of nucleic acids in Southern and Northern blotting workflows that require a durable PVDF support for immobilisation, washing and detection. The 0,45 µm porosity is commonly selected for standard nucleic-acid transfer applications and supports reliable retention during electrophoretic or capillary transfer. Pack size is 25 sheets with a weight of 0,075 kg, aligning with frequent mini-gel and cassette formats and reducing preparation time at the bench. This ROTI®ProBind PVDF membrane from manufacturer Carl ROTH is aimed at molecular-biology laboratories that value mechanical strength and chemical resistance in blotting consumables. Pre-cut sheets simplify setup, limit waste and help keep transfer conditions consistent across replicate runs. Typical use includes blotting applications and transfer of nucleic acids to membranes under standard laboratory practice.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 25 sheets
- Membrane type: PVDF transfer membrane
- Material: Polyvinylidene fluoride (PVDF)
- Pore size: 0,45 µm
- Dimensions: 10 × 10 cm
- Weight: 0,075 kg
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Questions about Transfer membrane ROTI® ProBind PVDF 0.45, 10 × 10 cm
What is this article?
Transfer membrane ROTI® ProBind PVDF 0.45, 10 × 10 cm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiology lab supplies.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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