{"product_id":"deae-dextran-4198","title":"DEAE Dextran, for biochemistry, 25 g, plastic","description":"\u003cp\u003eDEAE Dextran, for biochemistry, Molar mass (M) ~500000 g\/mol, CAS No. 9015-73-0\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eDEAE is the oldest transfection reagent known. The method of complexation of plasmid DNA with diethylaminoethyldextran has been published in 1968 by McCutchan. Positively charged DEAE binds negatively charged DNA to aggregates, which then bind to negtively charged surface structures of cell membranes. Uptake is done via endocytosis. This DEAE method needs only little amounts of DNA, while resulting in high transfection efficiency of up to 30 %. However, the cytotoxic effects caused by DEAE allow only transient transfections. The following cell lines are well transfectable: CV-1, BSC-1 and COS cells, and other stabile, insensitive cell lines.Stock solution: 50 mg\/ml in H\u003csub\u003e2\u003c\/sub\u003eO. Autoclave.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAppearance\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tweißes bis leicht gelbliches Pulver \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/SPEZ-4198-EN.pdf?context=bWFzdGVyfHNwZWNpZmljYXRpb25zfDEwMjE3MXxhcHBsaWNhdGlvbi9wZGZ8YURVekwyZ3pZaTg1TWpVME9EY3dPVEE0T1RVNEwxTlFSVnBmTkRFNU9GOUZUaTV3WkdZfDE1MDc3NjlhYjliZDA1MDY5NDlmNDBhYTg5ZWFkNDkxMjYyOTMzMjUzMzQ1M2Y1NjE5MTg3MTZhMTA3YWI3Y2Q\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"25 g \/ 1 piece","offer_id":56157862986060,"sku":"4198.1","price":80.1,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 6+ pieces","offer_id":56157863018828,"sku":"4198.1","price":76.1,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 24+ pieces","offer_id":56157863051596,"sku":"4198.1","price":72.09,"currency_code":"EUR","in_stock":true},{"title":"100 g \/ 1 piece","offer_id":56157863084364,"sku":"4198.2","price":263.4,"currency_code":"EUR","in_stock":true},{"title":"100 g \/ 6+ pieces","offer_id":56157863117132,"sku":"4198.2","price":250.23,"currency_code":"EUR","in_stock":true},{"title":"100 g \/ 24+ pieces","offer_id":56157863149900,"sku":"4198.2","price":237.06,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1000Wx1000H-Webshop-Platzhalter-Chem-LS_c1abda08-fa55-4a24-8a03-eaffecdc9029.jpg?v=1766439351","url":"https:\/\/www.luminixhealth.com\/en-nl\/products\/deae-dextran-4198","provider":"Luminix Health","version":"1.0","type":"link"}