{"product_id":"iptg-min-99-2316","title":"IPTG, min. 99 %, BioScience-Grade, dioxane-\/animal-free, 250 mg, glass","description":"\u003cp\u003eIPTG, min. 99 %, BioScience-Grade, dioxane-\/animal-free, Molar mass (M) 238,30 g\/mol, Flash point (flp) \u0026gt;110 °C, Storage temp. -20 °C, CAS No. 367-93-1, EC No. 206-703-0, Empirical formula C\u003csub\u003e9\u003c\/sub\u003eH\u003csub\u003e18\u003c\/sub\u003eO\u003csub\u003e5\u003c\/sub\u003eS\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eLactose analogon. Glucose-galactose-disaccharide. \u003ci\u003elac\u003c\/i\u003e-promotor is induced by IPTG via inhibition of \u003ci\u003elac\u003c\/i\u003e-repressor. Recommended for induction of \u003ci\u003elac\u003c\/i\u003e-regulated vectors in expression assays, for instance blue-\/white selection of recombinant \u003ci\u003eE. coli\u003c\/i\u003e, in bench-top as well as in production scale.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eBeing a galacto pyranoside, IPTG inhibits the lac repressor, therefore leading to the induction the lac promotor. Subsequently, using the plasmid as matrix the C-terminal 146 amino acid fragment of the bacterial β-galactosidase is expressed, which functions as so called α-donor and complements the C-terminally deleted bacterial β-galactosidase. The then functional β-galactosidase restricts the galactoside X-Gal, resulting in a blue-coloured dye - therefore, these lac+ clones are coloured dark blue. Since the C-terminal part of the α-donor is located \u003cb\u003eupstream\u003c\/b\u003e and the N-terminal part is located \u003cb\u003edownstream\u003c\/b\u003e of the multi cloning site of the plasmid, inserted DNA normally leads to frame shift or at least to major chain elongation, resulting in an unfunctional α-donor and no functional galactosidase - recombinant clones are white. The appearance of light blue clones either results form very short insertions or from the fact that in some plasmids the C-terminal fragment cloned upstream of the MCS is sufficient for α-complementation and restriction of a small amount of X-Gal.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eStock solution: 0,5 M (1 g \/ 8,4 ml) in water (sterilise through 0,2 µm filter).Final concentration in agar plates: 0,2 mM (add after autoclaving and cooling medium).Final concentration in induction medium: 2 mM.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-BlueWhiteSelection-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3wzMjQ5NjJ8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyaGxOeTlvTmpNdk9URXhOamN6T0RrM056Z3lNaTV3WkdZfDg1MmIwOTQ2ZDIyN2Y3YzgzMzFiNzA3NjdhZmE5NDFjNTlmNWY4MWU0ODZmOTA4MjJmOTU4NmNiNmJkYjQ1OTA\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"250 mg \/ 1 piece","offer_id":56127944458572,"sku":"2316.1","price":26.35,"currency_code":"EUR","in_stock":true},{"title":"250 mg \/ 6+ pieces","offer_id":56127944491340,"sku":"2316.1","price":25.03,"currency_code":"EUR","in_stock":true},{"title":"250 mg \/ 24+ pieces","offer_id":56127944524108,"sku":"2316.1","price":23.72,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 1 piece","offer_id":56127944556876,"sku":"2316.2","price":63.35,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 6+ pieces","offer_id":56127944589644,"sku":"2316.2","price":63.35,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 24+ pieces","offer_id":56127944622412,"sku":"2316.2","price":63.35,"currency_code":"EUR","in_stock":true},{"title":"5 g \/ 1 piece","offer_id":56127944655180,"sku":"2316.3","price":177.4,"currency_code":"EUR","in_stock":true},{"title":"5 g \/ 6+ pieces","offer_id":56127944687948,"sku":"2316.3","price":168.53,"currency_code":"EUR","in_stock":true},{"title":"5 g \/ 24+ pieces","offer_id":56127944720716,"sku":"2316.3","price":159.66,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 1 piece","offer_id":56127944753484,"sku":"2316.4","price":596.65,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 6+ pieces","offer_id":56127944786252,"sku":"2316.4","price":566.82,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 24+ pieces","offer_id":56127944819020,"sku":"2316.4","price":536.99,"currency_code":"EUR","in_stock":true},{"title":"100 g \/ 1 piece","offer_id":56127944851788,"sku":"2316.5","price":1553.4,"currency_code":"EUR","in_stock":true},{"title":"100 g \/ 6+ pieces","offer_id":56127944884556,"sku":"2316.5","price":1475.73,"currency_code":"EUR","in_stock":true},{"title":"100 g \/ 24+ pieces","offer_id":56127944917324,"sku":"2316.5","price":1398.06,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/2316-1000Wx1000H_3155c44d-8027-409e-bbb3-e8786abd8197.jpg?v=1766411877","url":"https:\/\/www.luminixhealth.com\/en-nl\/products\/iptg-min-99-2316","provider":"Luminix Health","version":"1.0","type":"link"}