{"product_id":"anti-c-myc-tag-mouse-monoclonal-1nnt-1nnt","title":"souris anti-c-myc-tag, monoclonale, 9E10, après dissolution dans 100 µl PBS, 100 µl","description":"\u003cp\u003esouris anti-c-myc-tag, monoclonal, 9E\u003csub\u003e10\u003c\/sub\u003e, après dissolution dans 100 µl de PBS, 250 µg\/ml, purifié par affinité (fabriqué par PROGEN), Densité (D) ~1 g\/cm³, Point d'ébullition (pb) ~100 °C, Température de stockage +4 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eAnticorps primaires spécifiques reconnaissant les tags protéiques les plus couramment utilisés. Les anticorps peuvent être utilisés de manière optimale dans des études de biologie cellulaire ou tissulaire, des essais d'interaction protéine-protéine, des études de modifications post-traductionnelles, de dynamique d'expression ou de localisation, jusqu'à la production de kits analytiques à l'échelle du laboratoire ou à grande échelle.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eDisponible pour les tags protéiques les plus couramment utilisés (His, DDDDK, GST, HA, myc, GFP \u0026 Strep)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHaute affinité pour le tag protéique respectif\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eDétection robuste et hautement spécifique de la protéine de fusion\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eNon conjugué\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eConvient par exemple pour le western blot, l'immunoprécipitation, l'ELISA et l'immunofluorescence\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\u003c!-- Base product name --\u003e\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eL'anticorps monoclonal 9E10 reconnaît le c-myc humain, un facteur de transcription de 62 kDa. Un tag c-myc est couramment ajouté aux protéines recombinantes et peut être utilisé pour la détection ou la purification de la protéine taggée.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eApplication\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eImmunoprécipitation (IP) : dépend de l'essai. Western Blot (WB) : 1:50 000-1:100 000 (0,005-0,0025 µg\/ml)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003eAnalyse Western blot de lysat d'E. coli contenant une protéine taggée c-myc avec un anticorps anti-c-myc-tag. L'analyse Western blot a été réalisée sur 80 ng, 40 ng, 10 ng ou 5 ng de lysat d'E. coli contenant une protéine taggée c-myc. Les cellules ont été lysées avec un tampon d'échantillon SDS. La membrane PVDF a été bloquée avec 5% de lait sec dans du PBST pendant 1 h à température ambiante. L'anticorps primaire anti-c-myc-tag souris monoclonal, 9E10 (N° de cat. 910MYCL) a été dilué dans le tampon de blocage (concentration d'anticorps 0,005 µg\/ml) et incubé pendant 1 h à température ambiante. L'anticorps secondaire chèvre anti-souris IgG polyclonal, conjugué HRP a également été dilué dans le tampon de blocage (concentration d'anticorps 0,2 µg\/ml) et incubé pendant 1 h à température ambiante. Les bandes ont été visualisées par détection chimiluminescente à l'aide du substrat de Western Blot Pierce™ ECL.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003ePas un dispositif médical \/ Pas un produit IVD\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eInformations techniques\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tIP WB \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eEspèce hôte\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tsouris \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eClonalité\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tmonoclonal \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003ePurification\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tChromatographie d'affinité \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eConjugué\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tonconjugé \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eIsotype\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tIgG1 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eClone\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t9E10 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eCible\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tc-myc-Tag \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n\u003ca download=\"\" href=\"https:\/\/www.carlroth.com\/medias\/BA-ART-1807893-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w5MDcwMzV8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmd3TlM5b01qTXZPVEEyTlRjd05ERTVOREEzT0M1d1pHWXwwNzg2ZmQ4MzFkZTU2ODAzZWIzMDA3MDFhNzcwMTljZmRlOWQ2NjA4ODA0MzgzNzU2NjhhNWU0ZDJkODNhYjU3\" rel=\"noopener noreferrer\" style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\" target=\"_blank\"\u003e\n\u003csvg aria-hidden=\"true\" style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n\u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n\u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n\u003ctext fill=\"currentColor\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" text-anchor=\"middle\" x=\"192\" y=\"380\"\u003ePDF\u003c\/text\u003e\n\u003c\/svg\u003e\n    Documentation technique (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"25 µg \/ 1 piece","offer_id":56125364404556,"sku":"1NNT.1","price":123.65,"currency_code":"EUR","in_stock":true},{"title":"25 µg \/ 6+ pieces","offer_id":56125364437324,"sku":"1NNT.1","price":117.47,"currency_code":"EUR","in_stock":true},{"title":"25 µg \/ 24+ pieces","offer_id":56125364470092,"sku":"1NNT.1","price":111.29,"currency_code":"EUR","in_stock":true},{"title":"100 µg \/ 1 piece","offer_id":56125364502860,"sku":"1NNT.2","price":342.95,"currency_code":"EUR","in_stock":true},{"title":"100 µg \/ 6+ pieces","offer_id":56125364535628,"sku":"1NNT.2","price":325.8,"currency_code":"EUR","in_stock":true},{"title":"100 µg \/ 24+ pieces","offer_id":56125364568396,"sku":"1NNT.2","price":308.66,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1000Wx1000H-1NNP_f685897d-e4d6-4d96-92df-538ec62a316d.jpg?v=1766403646","url":"https:\/\/www.luminixhealth.com\/fr-be\/products\/anti-c-myc-tag-souris-monoclonal-1nnt-1nnt","provider":"Luminix Health","version":"1.0","type":"link"}