Description
HPLC column NUCLEODUR®, 100-3 C18 ec, 250x4 mm
Material: stainless steel.
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Ideal and reliable standard RP phase for daily routine analyses and upscaling for preparative HPLC. Octadecyl (C18) and Octyl (C8) medium density with a pore size of 110 Å with exhaustive endcapping for a wide range of applications. Octadecyl (C18) and butyl (C4) with a pore size of 300 Å for the separation of biomolecules
Application examples:
- 110 Å: basic, neutral or acidic drugs; derivatized amino acids; pesticides; fat-soluble vitamins; aldehydes and ketones; phenolic compounds
- 300 Å: biomolecular macromolecules, like proteins and peptides
Delivery incl. 1x analytical HPLC column, instruction leaflet, certificate of analysis.
Technical Information
| Particle size | 3 µm |
| Pore size | 110 Å |
| L x Ø internal (column) | 250 x 4 mm |
| Column volume | 3.15 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | C18 ec |
| Surface chemistry | Medium density monomeric octadecyl (C18, ODS) modification |
| USP listing | L1 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | hydrophobic (van der Waals interactions), some residual silanol interactions |
| Pressure stability | 600 bar |
| pH stability | 1,0-9,0 |
| Recommended application(s) | Routine analyses Aldehydes Basic, neutral or acidic drugs Derivatised amino acids |
| Similar phases | NUCLEOSIL® C18 Spherisorb® ODS II Symmetry® C18 Hypersil™ ODS Inertsil® ODS II Kromasil® C18 LiChrospher® RP18 |
| Application Database (Macherey-Nagel) | HPLC SPE |