{"product_id":"lysozyme-min-45-000-fip-u-mg-8259","title":"Lysozyme, min. 45.000 FIP U\/mg,, lyophilised, 1 g, glass","description":"\u003cp\u003eLysozyme, min. 45.000 FIP U\/mg,, lyophilised, Molar mass (M) ~14000 g\/mol, Storage temp. -20 °C, CAS No. 12650-88-3, EC No. 235-747-3\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eSalt and albumin free lyophilised powder. Quality product for molecular biology. Ideal for lysis of bacteria for preparing DNA.Lysozyme preferentially hydrolyses the β-1,4-glycosidic bond between N-acetylmuraminic acid and N-acetylglucosamine. Muramic acid and acetylglucosamine are components of the proteoglycan cell wall of many microorganisms. The enzyme is mostly derived from chicken egg white and may efficiently be used for lysis of Gram+ bacteria. In genetic engineering, it is very often used during isolation of plasmid DNA or cellular RNA for the lysis of \u003ci\u003e E. coli \u003c\/i\u003eor eukaryotic cells.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eUnit definition\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e1 FIP-Unit equals the amount of enzyme which reduces the absorbance by 0.001 per min. at 450 nm, 25 °C, pH 7.00 using a suspension of FIP \u003ci\u003eMicrococcus luteus\u003c\/i\u003e as substrate (acc. to \u003ci\u003ePharmaceutical Enzymes\u003c\/i\u003e 1997). 1 FIP-Unit resembles approximately 1 Shugar-Unit.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eWorking solution: 5-10 mg\/ml (bacteria), 1-2 mg\/ml (eukaryotic cells)Buffer: salt solution without detegents (0.1 M potassium phosphate buffer, 50 mM Tris buffer, 1x PBS, 1x TBS or similar)pH optimum: 6.0-7.0Inhibitors: imidazole, indole, N-acetyl glucosamineTemperature optimum: 37 °CStability: The lyophilisate is stable at +4 °C and -20 °C for several years. The stability of the solution at pH 4-5 is several weeks at +4 °C and several days at room temperature.Isoelectric point: approx. 11Lysozyme solution should be frozen only once.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAdditional info\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tSource: Egg white \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/SPEZ-8259-EN.pdf?context=bWFzdGVyfHNwZWNpZmljYXRpb25zfDEwNDk4N3xhcHBsaWNhdGlvbi9wZGZ8YUROaUwyaGhOeTg1TWpVME9UTTRNVGd4TmpZeUwxTlFSVnBmT0RJMU9WOUZUaTV3WkdZfGY1NmE4ODk0ZjY3ZDMxYjE5MTdkMDU4NDRlODdmYTRmZWYwZTQxYTM1YzVhMzVmZTAxOGVjMTIxYmUwNzBiMzI\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 g \/ 1 piece","offer_id":56173275939148,"sku":"8259.1","price":40.75,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 6+ pieces","offer_id":56173275971916,"sku":"8259.1","price":40.75,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 24+ pieces","offer_id":56173276004684,"sku":"8259.1","price":40.75,"currency_code":"EUR","in_stock":true},{"title":"10 g \/ 1 piece","offer_id":56173276037452,"sku":"8259.2","price":206.95,"currency_code":"EUR","in_stock":true},{"title":"10 g \/ 6+ pieces","offer_id":56173276070220,"sku":"8259.2","price":196.6,"currency_code":"EUR","in_stock":true},{"title":"10 g \/ 24+ pieces","offer_id":56173276102988,"sku":"8259.2","price":186.26,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 1 piece","offer_id":56173276135756,"sku":"8259.3","price":418.2,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 6+ pieces","offer_id":56173276168524,"sku":"8259.3","price":397.29,"currency_code":"EUR","in_stock":true},{"title":"25 g \/ 24+ pieces","offer_id":56173276201292,"sku":"8259.3","price":376.38,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/8259-1000Wx1000H_442be641-cd1b-44ed-9429-36ab8bec56e9.jpg?v=1766443542","url":"https:\/\/www.luminixhealth.com\/fr-be\/products\/lysozyme-min-45-000-fip-u-mg-8259","provider":"Luminix Health","version":"1.0","type":"link"}