{"product_id":"roti-load-dnastain-1-green-1cn5-1cn5","title":"ROTI®Load DNAstain 1 Green,, tampon de chargement pour gel d'ADN 6x, prêt à l'emploi, 1,8 ml","description":"\u003cp\u003eROTI®Load DNAstain 1 Green, tampon de dépôt d'ADN 6x pour gel, prêt à l'emploi, sans DNase, densité (D) ~1,12 g\/cm³, température de stockage -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eChargement du tampon avec le colorant fluorescent SYBR\u003csup\u003e®\u003c\/sup\u003e Green I pour la coloration des acides nucléiques double brin dans les gels d'agarose et de polyacrylamide.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eAlternative au bromure d'éthidium, non toxique, non mutagène\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e20 fois plus sensible que le bromure d'éthidium, jusqu'à 0,01 ng d'acide nucléique par bande\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eÀ utiliser avec les filtres photo courants pour bromure d'éthidium à large bande\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eExcitation possible via la lumière UV (254 nm) et la lumière bleue (495 nm)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCompatible avec toutes les applications en aval habituelles.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eCharger DNAstain SYBR\u003csup\u003e®\u003c\/sup\u003e Green est facile à utiliser et réduit considérablement le temps nécessaire à l'analyse des gels. Le colorant fluorescent SYBR contenu\u003csup\u003e®\u003c\/sup\u003e Le SYBR Green I détecte jusqu'à 0,01 ng par bande et est, par conséquent, 20 fois plus sensible que le bromure d'éthidium. L'intégrité de l'ADN n'est pas influencée par le SYBR\u003csup\u003e®\u003c\/sup\u003e Green I ; par conséquent, l'ADN élué du gel peut être directement utilisé pour des applications en aval standard, telles que la ligation, la PCR, la transformation, la transfection, etc., sans effets interférents.SYBR\u003csup\u003e®\u003c\/sup\u003e Le Green I se lie au petit sillon de l'ADN et s'intercale dans l'ADN. Il peut être excité par la lumière UV et la lumière bleue, ce qui le rend parfaitement compatible avec les transilluminateurs UV standard ainsi qu'avec l'éclairage moderne à lumière bleue (voir aussi ROTIPHORESE\u003csup\u003e®\u003c\/sup\u003ePROfessional runVIEW, Réf. art. 4849.1). Lorsqu'il est excité, le SYBR lié aux acides nucléiques\u003csup\u003e®\u003c\/sup\u003e Le Green I émet une fluorescence verte aux couleurs vives (521 nm) qui peut être documentée par tous les dispositifs habituels à large bande pour le bromure d'éthidium ou le SYBR\u003csup\u003e®\u003c\/sup\u003e Filtres photo verts. Le \u003cb\u003efigure\u003c\/b\u003e représente respectivement 50 ng d'ADN par bande, ou 75 ng (1000 pb et 2,5 kb). Chaque piste a été chargée et colorée simultanément en utilisant 1 µl de ROTI\u003csup\u003e®\u003c\/sup\u003eCharger DNAstain SYBR\u003csup\u003e®\u003c\/sup\u003e Vert.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eWavelength\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eExcitation maximale\u003c\/b\u003e (lié à l'ADN) : 254 nm et 495 nm\u003cb\u003eÉmission maximale\u003c\/b\u003e (lié à l'ADN) : 521 nm\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\u003c!-- Base product name --\u003e\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eConseils d'utilisation\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eAfin de couvrir la principale gamme de tailles requise en électrophorèse sur gel, ROTI\u003csup\u003e®\u003c\/sup\u003eLoad DNAstain 1 Green contient du bleu de bromophénol et du xylène cyanol.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eLa glycérine est le réactif standard pour augmenter la densité des échantillons.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eRecommandé pour la coloration de fragments d'ADN double brin et d'ARN double brin de plus de 500 bp.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eInformations techniques\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eExcitation max. (lié à l'ADN)\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t~320 nm470-500 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eNombre de traces\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tenv. 1000\/ml \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eÉmissions max. (liées à l'ADN)\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t525 nm \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eTeinture\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tBleu de bromophénol, xylène cyanol, BrightGreen \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eMarquage de l'ADN inclus\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\toui \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eSensibilité\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t0,01 ng\/band\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eUtiliser\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tColoration simultanée du gel, fragments plus volumineux \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n\u003ca download=\"\" href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-GelStaining-NucleicAcids-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3w1ODg0NjJ8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyaGtNeTlvTVRBdk9URXhOamN6T1RVd01qRXhNQzV3WkdZfGEzZGRjODc4OTYwOTk2MDhhYjAyYmYzNWZiNzI5MzE0NDRhNTZmMmUzNDgzYWQ4NWQyZDE3OTQ4MjM4NjZjNjE\" rel=\"noopener noreferrer\" style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\" target=\"_blank\"\u003e\n\u003csvg aria-hidden=\"true\" style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n\u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n\u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n\u003ctext fill=\"currentColor\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" text-anchor=\"middle\" x=\"192\" y=\"380\"\u003ePDF\u003c\/text\u003e\n\u003c\/svg\u003e\n    Documentation technique (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1.8 ml \/ 1 piece","offer_id":56124299444556,"sku":"1CN5.1","price":56.9,"currency_code":"EUR","in_stock":true},{"title":"1.8 ml \/ 6+ pieces","offer_id":56124299477324,"sku":"1CN5.1","price":56.9,"currency_code":"EUR","in_stock":true},{"title":"1.8 ml \/ 24+ pieces","offer_id":56124299510092,"sku":"1CN5.1","price":56.9,"currency_code":"EUR","in_stock":true},{"title":"9 ml \/ 1 piece","offer_id":56124299542860,"sku":"1CN5.2","price":177.4,"currency_code":"EUR","in_stock":true},{"title":"9 ml \/ 6+ pieces","offer_id":56124299575628,"sku":"1CN5.2","price":168.53,"currency_code":"EUR","in_stock":true},{"title":"9 ml \/ 24+ pieces","offer_id":56124299608396,"sku":"1CN5.2","price":159.66,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/Gelbild-gruen-02-1000Wx1000H_86b69ac6-1d19-4843-8605-0e0be3f59309.jpg?v=1766402227","url":"https:\/\/www.luminixhealth.com\/fr-be\/products\/roti-charge-adn-teinte-1-vert-1cn5-1cn5","provider":"Luminix Health","version":"1.0","type":"link"}