{"product_id":"roti-fect-plus-cl21-cl21","title":"ROTI®Fect Plus, ready-to-use, CELLPURE®, 0.2 ml, plastic","description":"\u003cp\u003eROTI®Fect Plus, ready-to-use, CELLPURE®, for transfection, Density (D) ~1 g\/cm³, Storage temp. +4 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eHighly sophisticated transfection reagent for liposoma mediated transfection of eukaryotic cell lines and primary cells in culture. ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect PLUS further reduces the toxic effects of transfection, making it a versatile tool for transfection of sensitive cell lines or primary cells.Based on this, ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect PLUS is also especially recommended for transfection prior to \u003ci\u003ein vivo\u003c\/i\u003e analyses (siRNA-Assays, \u003ci\u003ein vivo\u003c\/i\u003e life microscopy, etc.), since the transfection stress is minimized, reducing stress dependent effects like the rate of apoptosis induced cells. Additionally, the stability of nucleic acid\/lipid complexes is diminished in comparison with ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect, therefore improving the release of nucleic acids in the cell.Thus, the newly established liposoma formulation of ROTI\u003cb\u003e\u003csup\u003e®\u003c\/sup\u003e\u003c\/b\u003eFect PLUS guarantees gentle and highly efficient transfections, even when hard-to-transfect or particularly sensitive cell lines are handled.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eParticularly low cell toxicity \u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSuperior transfection efficiency with all cell lines\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eEspecially recommended for hard-to-transfect cell lines\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eParticularly suited for use with primary cells\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eExcellent reproducibility\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eNo serum inhibition\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eSuccessfully tested on a variety of cell types.\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003esiRNA:\u003c\/b\u003e 1BR3, HEK293, HeLa, LN-308, T98G. U87MG, UACC-257\u003cb\u003ePrimary cells:\u003c\/b\u003e 1BR3, 8VM, CGN, HMEC, HUVEC, PBMC, S15VM\u003cb\u003eCell lines:\u003c\/b\u003e 1BRneo, 143B, 293T, NSCLC, alphaTC1, B16-F10, BHK-21, C2C12, C6, CHO, CHO-K1, Colo205, COS-7, EVLC2, HaCaT, HAEC, HCT116, HEK293, HeLa, HepG2, Hep 3B, IMR32, Jurkat, Kelly, KG1, La1-5S, LN-308, M2-10B4, MDCK, MEF, MG-63, MIN6, MLEC32, mPAC, mpkCCD, N18TG2, N2a, N-Tera2, NG NIH-3T3, Panc-1, PC 12, Phi-NX, Raji, RAW264.7, RD, RF24, RGE, S2, S91, SMA-560, SK-N-BE, SW480, T98G, U-2 OS, Vero, \u003ci\u003eet al.\u003c\/i\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eApprox. 400 assays per ml in 6 wells. Dependent on the cell line used.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure:\u003c\/b\u003eVia ROTI\u003csup\u003e®\u003c\/sup\u003eFect PLUS transfected cells.\u003cb\u003eA:\u003c\/b\u003e Fluorescent light (520 nm), \u003cb\u003eB:\u003c\/b\u003e Standard light.\u003cb\u003eAbove:\u003c\/b\u003e COS7 cells (0.5 × 10\u003csup\u003e5\u003c\/sup\u003e cells \/ cm\u003csup\u003e2\u003c\/sup\u003e in 24-well). Transfection after 24 h using 0.9 μg pCMV-GFP + 3.6 μl ROTI\u003csup\u003e®\u003c\/sup\u003eFect PLUS (ratio 1:4). Analysis after 48 h.\u003cb\u003eBelow:\u003c\/b\u003e BHK cells (0.2 × 10\u003csup\u003e5\u003c\/sup\u003e cells \/ cm\u003csup\u003e2\u003c\/sup\u003e in 24-well). \u003c\/div\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure:\u003c\/b\u003eTransfection efficiency subject to ratio of DNA to ROTI\u003csup\u003e®\u003c\/sup\u003eFect PLUS. For most cell lines, the optimised composition of transfection complex is 1:3 to 1:4.Vero: 1.2 × 10\u003csup\u003e5\u003c\/sup\u003e cells \/ cm\u003csup\u003e2\u003c\/sup\u003e; MDCK: 1.2 × 10\u003csup\u003e5\u003c\/sup\u003e cells \/ cm\u003csup\u003e2\u003c\/sup\u003e; COS7: 5 × 10\u003csup\u003e4\u003c\/sup\u003e cells \/ cm\u003csup\u003e2\u003c\/sup\u003e; HeLa: 1 × 10\u003csup\u003e5\u003c\/sup\u003e cells \/ cm\u003csup\u003e2\u003c\/sup\u003e. Transfection with pCMV-LacZ 24 h after sawing in 48 wells in serum-free DMEM. Analysis (ONPG assay) after 48 h.\u003c\/div\u003e\n\n\n\n\n\u003cdiv\u003e\n\u003cb\u003eFigure:\u003c\/b\u003eTransfection efficiency of ROTI\u003csup\u003e®\u003c\/sup\u003eFect PLUS given in percentage. For standardisation, transfection efficiency of ROTI\u003csup\u003e®\u003c\/sup\u003eFect was set as reference (100 %).Compared to ROTI\u003csup\u003e®\u003c\/sup\u003eFect, transfection efficiency achieved by ROTI\u003csup\u003e®\u003c\/sup\u003eFect PLUS is at least equivalent. For some cell lines known to be hard-to-transfect with ROTI\u003csup\u003e®\u003c\/sup\u003eFect, e.g. Vero, CV1, or CHO-K1 cells, ROTI\u003csup\u003e®\u003c\/sup\u003eFect PLUS achieves multiple enhancement of transfection efficiency.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-TransfectionProtocols-CL21-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3w1NzYzMzZ8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyZzROeTlvTW1Vdk9URXhOamN6T1RBeE1EVTVNQzV3WkdZfGIzZjZhYzhkMTQwYTk1MzJiZDRiMjhlZGE0OWU1ZGZiN2VmMGE2ZjI0MWY2ZTM3NzFmZGNhYzg3N2E0YTE5NzI\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"0.2 ml \/ 1 piece","offer_id":56180020183372,"sku":"CL21.1","price":123.65,"currency_code":"EUR","in_stock":true},{"title":"0.2 ml \/ 6+ pieces","offer_id":56180020216140,"sku":"CL21.1","price":117.47,"currency_code":"EUR","in_stock":true},{"title":"0.2 ml \/ 24+ pieces","offer_id":56180020248908,"sku":"CL21.1","price":111.29,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 1 piece","offer_id":56180020281676,"sku":"CL21.2","price":446.15,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 6+ pieces","offer_id":56180020314444,"sku":"CL21.2","price":446.15,"currency_code":"EUR","in_stock":true},{"title":"1 ml \/ 24+ pieces","offer_id":56180020347212,"sku":"CL21.2","price":446.15,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1000Wx1000H-CL21_3e346531-7a46-47a0-8656-d80bb4f839c9.jpg?v=1766447566","url":"https:\/\/www.luminixhealth.com\/fr-be\/products\/roti-fect-plus-cl21-cl21","provider":"Luminix Health","version":"1.0","type":"link"}