{"product_id":"roti-free-stripping-buffer-2-0-3319","title":"ROTI®Free Stripping Buffer 2.0, ready-to-use, 250 ml, glass","description":"\u003cp\u003eROTI®Free Stripping Buffer 2.0, ready-to-use, Density (D) ~1 g\/cm³, Boiling point (bp) ~100 °C, Storage temp. +15 to +25 °C, UN-Nr. 1760\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eStripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki \u003ci\u003eet. al.\u003c\/i\u003e (1981) \u003ci\u003eAnalyt. Biochem.\u003c\/i\u003e 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eEffective\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGentle\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOdour-free\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSimple application w\/o heating\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCost-efficient\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFollowing rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI\u003csup\u003e®\u003c\/sup\u003eFree Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody\/epitope pairing.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/SPEZ-3319-EN.pdf?context=bWFzdGVyfHNwZWNpZmljYXRpb25zfDk1NzkyfGFwcGxpY2F0aW9uL3BkZnxhR1UxTDJobU1pODVNalUwT0RJeU5UUXpNemt3TDFOUVJWcGZNek14T1Y5RlRpNXdaR1l8MmVjM2M0OWU4ZTE3ZGUwZGQ4NDYzYjI5Y2JlODc1MGYyMTNiNTVmMjgyMzFhYTgzZTE4ZjI2YzQ5ZTQ5ZDM0NA\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"250 ml \/ 1 piece","offer_id":56149180219724,"sku":"3319.1","price":56.9,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 6+ pieces","offer_id":56149180252492,"sku":"3319.1","price":54.06,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 24+ pieces","offer_id":56149180285260,"sku":"3319.1","price":51.21,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 1 piece","offer_id":56149180318028,"sku":"3319.2","price":145.15,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 6+ pieces","offer_id":56149180350796,"sku":"3319.2","price":145.15,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 24+ pieces","offer_id":56149180383564,"sku":"3319.2","price":145.15,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/3319-1000Wx1000H_d484b897-1862-4a9a-b7db-91502780b9ca.jpg?v=1766434332","url":"https:\/\/www.luminixhealth.com\/fr-be\/products\/roti-free-stripping-buffer-2-0-3319","provider":"Luminix Health","version":"1.0","type":"link"}