{"product_id":"roti-free-strippingpuffer-2-2-plus-3337","title":"ROTI®Free StrippingPuffer 2.2 plus, ready-to-use, 250 ml, glass","description":"\u003cp\u003eROTI®Free StrippingPuffer 2.2 plus, ready-to-use, Density (D) 1,01 g\/cm³, Boiling point (bp) ~100 °C, Storage temp. +15 to +25 °C, UN-Nr. 1760\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eStripping buffer for removal of high-affinity antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki \u003ci\u003eet. al.\u003c\/i\u003e (1981) \u003ci\u003eAnalyt. Biochem.\u003c\/i\u003e 111:385-92). Specially designed additives enhance the efficiency in removing those antibodies requiring long stripping times or high incubation temperatures with standard stripping buffer systems. Advanced formulation without β-mercaptoethanol or DTT for use at room temperature or heated. Suitable for nitrocellulose and PVDF membranes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVery effective\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eGentle\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOdour-free\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSimple application\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eAlso suitable for high-affinity antibodies\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eFollowing rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI\u003csup\u003e®\u003c\/sup\u003eFree Stripping-Puffer 2.2 plus for 30-60 mins. at room temperature. In case of very persistent antibodies incubation in heated buffer is allowed. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody\/epitope pairing.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/SPEZ-3337-EN.pdf?context=bWFzdGVyfHNwZWNpZmljYXRpb25zfDk1ODQ0fGFwcGxpY2F0aW9uL3BkZnxhR1l6TDJnNU9DODVNalUwT0RJek9EVTBNVEV3TDFOUVJWcGZNek16TjE5RlRpNXdaR1l8ZWYyMzgxZjkxOTg3ZTdlYTc5YjY3OTU4MDE0NDVmMjk1NTQyZGM2ZmE5YmZhZDA2NzhjNTBmNjFkNTk1YjBiYg\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"250 ml \/ 1 piece","offer_id":56149207417164,"sku":"3337.1","price":66.55,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 6+ pieces","offer_id":56149207449932,"sku":"3337.1","price":63.22,"currency_code":"EUR","in_stock":true},{"title":"250 ml \/ 24+ pieces","offer_id":56149207482700,"sku":"3337.1","price":59.9,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 1 piece","offer_id":56149207515468,"sku":"3337.2","price":160.2,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 6+ pieces","offer_id":56149207548236,"sku":"3337.2","price":160.2,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 24+ pieces","offer_id":56149207581004,"sku":"3337.2","price":160.2,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/3337-1000Wx1000H_c240a40a-2ba2-4623-a605-bf4987ed2a6a.jpg?v=1766434354","url":"https:\/\/www.luminixhealth.com\/fr-be\/products\/roti-free-strippingpuffer-2-2-plus-3337","provider":"Luminix Health","version":"1.0","type":"link"}