Description
ROTI®Prep Plant RNA, 10 pcs, for molecular biology, Density (D) 1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep Plant RNA is designed to isolate total RNA from various plant starting materials.The isolation is done from homogenised plant material, which is then treated with one of the lysis buffers included in the kit to break down even complex plant components. The lysed sample is then filtered through a centrifugation column, which binds the DNA. The filtrate is then transferred to a second centrifugation column that binds the RNA. After several washing and centrifugation steps, the RNA is eluted from the membrane by the elution buffer and is now ready for use in all subsequent applications.
- Specially developed for extraction from plant materials
- Preparation by well-known mini spin-column system
- Fast, easy and efficient
- Preparation in approx. 30 min
- Yield up to 70 µg RNA, depending on source material
- Use of DNase not necessary
- Blossoms
- Leaves
- Roots
- Stems
- Wooden parts
- Seeds
- Grains
Lysis Buffer LSR, Lysis Buffer LSK, Washing Buffer WSA (conc.), Washing Solution WSL (conc.) RNase-free Water, centrifugation columns DNA (blue), centrifugation columns RNA (violet), collection tubes, elution tubes.Ethanol 99.8 % (p.a., Art. no. 9065) is not included in the kit and should be ordered separately if required.
| Basic material | Fresh, frozen, or dried plant material |
| Quantity of material | 100 mg |
| Preparation time | approx. 30 min |
| Instructions for use, addition | Before extraction, the plant material should first be homogenised in a ROTI®SampleLyse tube (Art. No. 1YKA.1), or by means of a mortar and pestle. |
| Version | Mini prep |
| Automatic use | no |
| Elution volume | 30-80 µl |
| Typical yield | <70 μg |
| Typical purity (A260/A280) | 1.8 - 2.1 |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)