Description
ROTI®Prep Soil DNA, 10 pieces, for molecular biology, Density (D) ~1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep Soil DNA was developed to isolate microbial DNA from soil samples.Soil samples represent a particularly complex starting material, which is why it is urgently necessary to first mechanically decompose it. In the first step of extraction, the soil samples are homogenised and disrupted in a lysis tube in lysis buffer. After a centrifugation step, the lysate is transferred to the centrifugation column and the DNA is bound to the membrane with the aid of the binding buffer. After several washing and centrifugation steps, the DNA is then eluted from the membrane with the elution buffer.
- Specially developed for DNA extraction from complex soil samples
- Preparation in the well-established mini-column system
- Fast, easy and reliable
- Extraction time of approx. 45 min
- Soil sample (up to 400 mg)
ROTI®SampleLyse Microbes 1 tubes, Lysis Buffer LSS, Precipitation Buffer PB, Binding Buffer BR, Binding Buffer BDS, Washing Solution WSO, Washing Buffer WSH (conc.), Elution Buffer EB, detailed instructions.Ethanol 99.8 % (p.a., Art. no. 9065) and a homogeniser (e.g. Art. no. PA66.1) are not included in the kit and should be ordered separately if required.
| Basic material | complex soil samples |
| Quantity of material | < 400 mg |
| Instructions for use, addition | During extraction, the soil sample is first mechanically disrupted in a homogeniser (e.g. order no. PA66.1) using a ROTI®SampleLyse tube. |
| Version | Mini prep |
| Automatic use | no |
| Elution volume | 50-100 µl |
| Typical yield | < 30 μg |
| Typical purity (A260/A280) | 1.7-2.0 |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)