Description
Le Semi Dry Blotter PROfessional MAXI est un système de transfert semi-sec grand format destiné au transfert d'acides nucléiques sur membranes lors de procédures de Southern et Northern blot. Il offre une surface de transfert de 20 × 20 cm, permettant d'accueillir des gels plus grands et un débit d'échantillons supérieur à celui des systèmes mini, tout en conservant l'encombrement réduit d'un appareil de paillasse. Le fonctionnement semi-sec limite la consommation de tampon par rapport aux systèmes de transfert immergés et permet un transfert rapide et contrôlé d'ADN ou d'ARN après électrophorèse horizontale sur agarose. L'unité est fournie comme un instrument complet de la gamme ROTIPHORESE® PROfessional, prêt à être intégré dans les flux de travail de biologie moléculaire. Les dimensions uniformes des plaques favorisent un contact constant avec la membrane sur toute la surface de transfert. Ce modèle MAXI du fabricant ROTH SELECTION convient aux laboratoires qui traitent régulièrement des gels plus grands ou plusieurs échantillons en parallèle. Il offre une solution de mise à l'échelle pratique par rapport aux mini-blotters pour les groupes de recherche axés sur la détection et la documentation d'acides nucléiques sur membranes.
Cet article appartient à Membranes et papiers de transfert, dans Biologie des protéines et Western blot.
Caractéristiques principales
- Conditionnement : 1 unité
- Type de produit : Blotter semi-sec
- Série : ROTIPHORESE® PROfessional MAXI
- Surface de transfert : 20 × 20 cm
- Technique : Transfert semi-sec (Southern/Northern)
- Fiche technique :
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Questions about Semi Dry Blotter PROfessional MAXI, ROTIPHORESE®, 1 unité(s)
What is this article?
Semi Dry Blotter PROfessional MAXI, ROTIPHORESE®, 1 unité(s) is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiologie.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiologie.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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