{"product_id":"roti-transform-for-prep-of-competent-p043-p043","title":"ROTI®Transform, for prep. of competent, E. coli, sterile, ready-to-use, 1 kit","description":"\u003cp\u003eROTI®Transform, for prep. of competent, E. coli, sterile, ready-to-use, Density (D) 1 g\/cm³, Storage temp. -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\u003cdiv\u003e\u003cb\u003eSterile\u003c\/b\u003e\u003c\/div\u003e\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eTransform is a simple, effective and fast method of producing competent \u003ci\u003eE. coli\u003c\/i\u003e in three steps. The competent cells may be used directly for transformation or may be frozen for later transformations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFlexible selection of strains\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eEffective, simple and fast\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eAdequate for 500 transformations\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eTransformation efficiency is 5 x 10\u003csup\u003e5\u003c\/sup\u003e to 1 x 10\u003csup\u003e6\u003c\/sup\u003e colonies per µg DNA.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eProtocol:\u003c\/b\u003e1. Resuspend cells in fresh, cold LB-medium2. Add ROTI\u003csup\u003e®\u003c\/sup\u003eTransform 13. Add ROTI\u003csup\u003e®\u003c\/sup\u003eTransform 2 and incubate for 5 minutes on ice.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eThe kit contains\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTI\u003csup\u003e®\u003c\/sup\u003eTransform consists of 45 ml ROTI\u003csup\u003e®\u003c\/sup\u003eTransform 1 (Art. No. P041) and 5 ml ROTI\u003csup\u003e®\u003c\/sup\u003eTransform 2 (Art. No. P042).\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eUser information\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eLong-term Storage\u003c\/b\u003e of the Kit at -20 °C.\u003cb\u003eStorage for use:\u003c\/b\u003eReagent 1: Short-term storage may be done at 4 °C. For long-term storage of over 14 days we recommend a storage temperature of -20 °C.Reagent 2: Storage at 4 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-P043-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3wzNzYyOTR8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmc0TlM5b1pEWXZPVEF6TnpjeU9ESTFNVGt6TkM1d1pHWXxlZGM2Nzc0YmJhYjk2Mjk0MGFiYTQ4ZWVhMDhjMzBlNDAzOGU3NGE4NGY1NWZhZjE5ZTExOWVlMTdmNWYzMWYz\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 piece \/ 1 piece","offer_id":56184724685132,"sku":"P043.1","price":84.85,"currency_code":"EUR","in_stock":true},{"title":"6+ pieces \/ 6+ pieces","offer_id":56184724717900,"sku":"P043.1","price":80.61,"currency_code":"EUR","in_stock":true},{"title":"24+ pieces \/ 24+ pieces","offer_id":56184724750668,"sku":"P043.1","price":76.37,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/P043-1000Wx1000H_d6aad491-5da4-45eb-a49f-ec14112d1733.jpg?v=1766493562","url":"https:\/\/www.luminixhealth.com\/fr-fr\/products\/roti-transform-for-prep-of-competent-p043-p043","provider":"Luminix Health","version":"1.0","type":"link"}