{"product_id":"rotiphorese-10x-3060","title":"ROTIPHORESE® 10x, SDS-PAGE, 10x conc. buffer concentrate, 1 l, glass","description":"\u003cp\u003eROTIPHORESE® 10x, SDS-PAGE, 10x conc. buffer concentrate, Density (D) 1,068 g\/cm³, Boiling point (bp) ~100 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 100%; max-width:100%; box-sizing:border-box; margin:10px 0; margin-left:0; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eConcentrated stock solution for tris\/glycine\/SDS gel run buffer (PAGE)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eStandardised composition according to Lämmli (\u003ci\u003eNature\u003c\/i\u003e)\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eMade from high-quality reagents\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eFor constant quality and highly reproducible gels\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eSDS\u003c\/b\u003e denatures proteins and binds in a constant mass ratio to their peptide chains, covering their intrinsic charge. In the collection gel (pH 6.8) all proteins are kept in a sharp band between the neutral \u003cb\u003eGlycin\u003c\/b\u003e and chloride ions and thus all reach the separation gel at the same time. After the pH change when the separation gel is reached (pH 8.8), the chloride ions and the now cationic \u003cb\u003eGlycin\u003c\/b\u003e migrate very quickly to the anode and leave the proteins to themselves, which now move in the electric field \u003cb\u003edepending on size\u003c\/b\u003e towards the anode.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTIPHORESE\u003csup\u003e®\u003c\/sup\u003e10x SDS-PAGE contains 0,25 M Tris, 1,92 M glycine and 1 % (w\/v) SDS in distilled, deionised water for protein analysis. Acc. to Laemmli (1970), \u003ci\u003eNature\u003c\/i\u003e 227:680.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eROTIPHORESE\u003csup\u003e®\u003c\/sup\u003e10x SDS-PAGEis usually used as 1x concentrated solution. SDS-PAGE buffer is suitable for the denaturing separation of proteins and peptides of any size in the discontinuous polyacrylamide gel (collection gel \/ separation gel).\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/SPEZ-3060-EN.pdf?context=bWFzdGVyfHNwZWNpZmljYXRpb25zfDk0ODY0fGFwcGxpY2F0aW9uL3BkZnxhRGswTDJnNU15ODVNalUwTnprd01EQTBOelkyTDFOUVJWcGZNekEyTUY5RlRpNXdaR1l8ZTQ2NWE1NDU1ZjBlN2ZiODI4ZDcxNDA3NWI3OThjMTA3ZTNmZTY3ZTE2YzA5NDg1MDQ3ODhiYzMwY2U3MWNlYw\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 liter \/ 1 piece","offer_id":56148411646284,"sku":"3060.1","price":50.45,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 6+ pieces","offer_id":56148411679052,"sku":"3060.1","price":50.45,"currency_code":"EUR","in_stock":true},{"title":"1 liter \/ 24+ pieces","offer_id":56148411711820,"sku":"3060.1","price":50.45,"currency_code":"EUR","in_stock":true},{"title":"2.5 liter \/ 1 piece","offer_id":56148411744588,"sku":"3060.3","price":91.3,"currency_code":"EUR","in_stock":true},{"title":"2.5 liter \/ 6+ pieces","offer_id":56148411777356,"sku":"3060.3","price":86.74,"currency_code":"EUR","in_stock":true},{"title":"2.5 liter \/ 24+ pieces","offer_id":56148411810124,"sku":"3060.3","price":82.17,"currency_code":"EUR","in_stock":true},{"title":"5 liter \/ 1 piece","offer_id":56148411842892,"sku":"3060.2","price":131.7,"currency_code":"EUR","in_stock":true},{"title":"5 liter \/ 6+ pieces","offer_id":56148411875660,"sku":"3060.2","price":125.12,"currency_code":"EUR","in_stock":true},{"title":"5 liter \/ 24+ pieces","offer_id":56148411908428,"sku":"3060.2","price":118.53,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/3060-1000Wx1000H.jpg?v=1766431870","url":"https:\/\/www.luminixhealth.com\/fr-fr\/products\/rotiphorese-10x-3060","provider":"Luminix Health","version":"1.0","type":"link"}