{"product_id":"x-beta-gal-2315","title":"X-beta-Gal, min. 99 %, BioScience-Grade, 100 mg, glass","description":"\u003cp\u003eX-beta-Gal, min. 99 %, BioScience-Grade, Molar mass (M) 408,6 g\/mol, Storage temp. -20 °C, CAS No. 7240-90-6, EC No. 230-640-8, Empirical formula C\u003csub\u003e14\u003c\/sub\u003eH\u003csub\u003e15\u003c\/sub\u003eBrClNO\u003csub\u003e6\u003c\/sub\u003e\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eIdeal for colorimetric detection of β-galactosidase activity, for instance during blue-\/white selection.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eMechanism\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eBeing a galacto pyranoside, IPTG inhibits the lac repressor, therefore leading to the induction the lac promotor. Subsequently, using the plasmid as matrix the C-terminal 146 amino acid fragment of the bacterial β-galactosidase is expressed, which functions as so called α-donor and complements the C-terminally deleted bacterial β-galactosidase. The then functional β-galactosidase restricts the galactoside X-Gal, resulting in a blue-coloured dye - therefore, these lac+ clones are coloured dark blue. Since the C-terminal part of the α-donor is located \u003cb\u003eupstream\u003c\/b\u003e and the N-terminal part is located \u003cb\u003edownstream\u003c\/b\u003e of the multi cloning site of the plasmid, inserted DNA normally leads to frame shift or at least to major chain elongation, resulting in an unfunctional α-donor and no functional galactosidase - recombinant clones are white. The appearance of light blue clones either results form very short insertions or from the fact that in some plasmids the C-terminal fragment cloned upstream of the MCS is sufficient for α-complementation and restriction of a small amount of X-Gal.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eWorking solution: for blue\/white selection of recombinant clones on agar plates use 0.3 μl stock solution per ml broth\/agar.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eEnzyme\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tβ-D-Galactosidase \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eSignal product\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tPrecipitate (blue\/white selection) \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-BlueWhiteSelection-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3wzMjQ5NjJ8YXBwbGljYXRpb24vcGRmfGRHVmphRzVwWTJGc1JHOWpkVzFsYm5SekwyaGxOeTlvTmpNdk9URXhOamN6T0RrM056Z3lNaTV3WkdZfDg1MmIwOTQ2ZDIyN2Y3YzgzMzFiNzA3NjdhZmE5NDFjNTlmNWY4MWU0ODZmOTA4MjJmOTU4NmNiNmJkYjQ1OTA\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"100 mg \/ 1 piece","offer_id":56127943934284,"sku":"2315.1","price":27.45,"currency_code":"EUR","in_stock":true},{"title":"100 mg \/ 6+ pieces","offer_id":56127943967052,"sku":"2315.1","price":26.08,"currency_code":"EUR","in_stock":true},{"title":"100 mg \/ 24+ pieces","offer_id":56127943999820,"sku":"2315.1","price":24.71,"currency_code":"EUR","in_stock":true},{"title":"500 mg \/ 1 piece","offer_id":56127944032588,"sku":"2315.2","price":81.2,"currency_code":"EUR","in_stock":true},{"title":"500 mg \/ 6+ pieces","offer_id":56127944065356,"sku":"2315.2","price":77.14,"currency_code":"EUR","in_stock":true},{"title":"500 mg \/ 24+ pieces","offer_id":56127944098124,"sku":"2315.2","price":73.08,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 1 piece","offer_id":56127944130892,"sku":"2315.3","price":145.15,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 6+ pieces","offer_id":56127944163660,"sku":"2315.3","price":145.15,"currency_code":"EUR","in_stock":true},{"title":"1 g \/ 24+ pieces","offer_id":56127944196428,"sku":"2315.3","price":145.15,"currency_code":"EUR","in_stock":true},{"title":"2.5 g \/ 1 piece","offer_id":56127944229196,"sku":"2315.5","price":278.45,"currency_code":"EUR","in_stock":true},{"title":"2.5 g \/ 6+ pieces","offer_id":56127944261964,"sku":"2315.5","price":264.53,"currency_code":"EUR","in_stock":true},{"title":"2.5 g \/ 24+ pieces","offer_id":56127944294732,"sku":"2315.5","price":250.61,"currency_code":"EUR","in_stock":true},{"title":"5 g \/ 1 piece","offer_id":56127944327500,"sku":"2315.4","price":504.2,"currency_code":"EUR","in_stock":true},{"title":"5 g \/ 6+ pieces","offer_id":56127944360268,"sku":"2315.4","price":478.99,"currency_code":"EUR","in_stock":true},{"title":"5 g \/ 24+ pieces","offer_id":56127944393036,"sku":"2315.4","price":453.78,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/2315-1000Wx1000H_ef20a5b9-3c7b-434a-8d44-68388223ff26.jpg?v=1766411875","url":"https:\/\/www.luminixhealth.com\/fr-fr\/products\/x-beta-gal-2315","provider":"Luminix Health","version":"1.0","type":"link"}