anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plastic - Luminix Health
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anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plastic - Luminix Health

anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 0.2 ml, plastic

SKU: 1NNE.1
Volume: 0.2 ml
Value Packs: 1 piece
Price:
€109,15

Description

anti-Cyclin-Dependent Kinase 4, mouse monoclonal, DCS-156, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

DCS-156 reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. In immunohistochemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) and cervix DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation. Epitope recognized: aa 270-290.

Application

Western Blot (WB): 1:50-1:500 (0.1-1 μg/ml)

Western blot analysis of human HeLa cell lysate with anti-Cyclin-Dependent Kinase 4 antibody. Western blot analysis was performed on either 5 μg or 10 μg of HeLa lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Cyclin-Dependent Kinase 4 mouse monoclonal, DCS-156 (Cat. No. 690096) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Not a medical device / Not an IVD product
Technical Information
Application WB 
Host species mouse 
Clonality monoclonal 
Reactivity antibody mouse human rat 
Purification Affinity chromatography 
Conjugate uncojugated 
Concentration 50 µg/ml 
Isotype IgG1 
Uniprot ID AK P11802 (human),P30285 (mouse),P35426 (rat) 
Clone DCS-156 
Target human recombinant full-length cdk4 polypeptide 

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