{"product_id":"roti-cell-trypsin-edta-solution-1x-1y1a-1y1a","title":"Solution Trypsine\/EDTA ROTI®Cell (1x), stérile, 1x, CELLPURE®, 100 ml, plastique","description":"\u003cp\u003eSolution de trypsine\/EDTA ROTI®Cell (1x), stérile, 1x, CELLPURE®, Densité (D) 1 g\/cm³, Temp. de stockage -20 °C\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\u003cdiv\u003e\u003cb\u003eStérile\u003c\/b\u003e\u003c\/div\u003e\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eLa trypsine est une enzyme digestive protéolytique du pancréas appartenant à la famille des sérine protéases, qui clive la lysine et l'arginine au niveau du C-terminus. Les solutions de trypsine sont utilisées pour séparer les cellules des tissus ou des monocouches cellulaires. Elles sont donc essentielles pour les applications de routine dans le passage de cultures cellulaires, pour les expériences de culture cellulaire afin d'obtenir un nombre suffisant de cellules ou pour la dissociation de tissus primaires. En alternative à la trypsine, la solution plus protectrice pour les cellules ROTI\u003csup\u003e®\u003c\/sup\u003eCell Accutase peut être utilisée, elle est exempte de composants mammifères.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\u003c!-- Base product name --\u003e\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eCette solution de trypsine contient de l'EDTA. L'EDTA est un agent chélatant qui améliore la capacité de la trypsine à détacher les cellules adhérentes. L'EDTA se lie au calcium et au magnésium, ce qui affaiblit davantage les contacts cellulaires. Ceci favorise l'hydrolyse de liaisons peptidiques spécifiques par la trypsine.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eLa concentration de trypsine requise dépend principalement du type de cellule et de l'âge de la culture cellulaire. À titre indicatif, vous trouverez un protocole complet dans la brochure d'informations techniques.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eAprès dissociation cellulaire, la trypsine doit être inactivée. Ceci peut être réalisé soit par l'utilisation de FCS, soit par des inhibiteurs de trypsine.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eInformations techniques\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eEnzyme\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tTrypsine de pancréas porcin \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAutre nom\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tRéactifs de dissociation cellulaire \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eUtilisation\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tDissociation cellulaire \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eInstructions d'utilisation, ajout\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tPréparation d'aliquotes recommandée pour éviter les cycles répétés de congélation-décongélation. \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n\u003ca download=\"\" href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-TrypsinCellPassage-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3wxOTkyOTF8YXBwbGljYXRpb24vcGRmfGFEVTJMMmhtTmk4NU1UYzBOamMzTmpnME1qVTBMMGx1Wm05ZlFuSnZZMmgxY21WZlZISjVjSE5wYmtObGJHeFFZWE56WVdkbFgwVk9MbkJrWmd8YWVkZmU5Y2Y0NTRhMTQ1MTczODVhOGM0NzMyOGIzZDFjZmI4YzEzODMzYTZhNDA2ZWNlODdiOTAyM2Q1MjI1MA\" rel=\"noopener noreferrer\" style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\" target=\"_blank\"\u003e\n\u003csvg aria-hidden=\"true\" style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n\u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n\u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n\u003ctext fill=\"currentColor\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" text-anchor=\"middle\" x=\"192\" y=\"380\"\u003ePDF\u003c\/text\u003e\n\u003c\/svg\u003e\n    Documentation technique (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1rem;\"\u003e\n\u003ca download=\"\" href=\"https:\/\/www.carlroth.com\/medias\/Info-Brochure-TrypsinCellPassage-EN.pdf?context=bWFzdGVyfHRlY2huaWNhbERvY3VtZW50c3wxOTkyOTF8YXBwbGljYXRpb24vcGRmfGFEVTJMMmhtTmk4NU1UYzBOamMzTmpnME1qVTBMMGx1Wm05ZlFuSnZZMmgxY21WZlZISjVjSE5wYmtObGJHeFFZWE56WVdkbFgwVk9MbkJrWmd8YWVkZmU5Y2Y0NTRhMTQ1MTczODVhOGM0NzMyOGIzZDFjZmI4YzEzODMzYTZhNDA2ZWNlODdiOTAyM2Q1MjI1MA\" rel=\"noopener noreferrer\" style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\" target=\"_blank\"\u003e\n\u003csvg aria-hidden=\"true\" style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n\u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n\u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n\u003ctext fill=\"currentColor\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" text-anchor=\"middle\" x=\"192\" y=\"380\"\u003ePDF\u003c\/text\u003e\n\u003c\/svg\u003e\n    Déclaration de conformité CE (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"100 ml \/ 1 piece","offer_id":56127477743948,"sku":"1Y1A.1","price":22.0,"currency_code":"USD","in_stock":false},{"title":"100 ml \/ 6+ pieces","offer_id":56127477776716,"sku":"1Y1A.1","price":21.0,"currency_code":"USD","in_stock":false},{"title":"100 ml \/ 24+ pieces","offer_id":56127477809484,"sku":"1Y1A.1","price":20.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/1Y1A-1000Wx1000H_5b0966b3-e29c-4711-8b55-77c12f46158a.jpg?v=1766408281","url":"https:\/\/www.luminixhealth.com\/fr\/products\/roti-cell-trypsin-edta-solution-1x-1y1a-1y1a","provider":"Luminix Health","version":"1.0","type":"link"}