{"product_id":"roti-prep-plasmid-mini-xl-8546","title":"ROTI®Prep Plasmide MINI-XL","description":"\u003cp\u003eROTI®Prep Plasmid MINI-XL, voor moleculaire biologie, Dichtheid (D) 1,05 g\/cm³, Opslagtemperatuur +15 tot +25 °C, UN-nr. 3316\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\u003c!--superDetailElements --\u003e\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\u003c!-- Base product name --\u003e\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003e\u003cb\u003eNieuw \u003c\/b\u003e-\u003cb\u003eMet geoptimaliseerd protocol voor isolatie uit 0,5-2,0 ml bacteriecultuur\u003c\/b\u003e!\u003cb\u003eMIDI-preps in MINI-formaat.ROTI\u003c\/b\u003e\u003csup\u003e®\u003c\/sup\u003eROTI®Prep Plasmid MINI-XL is een verdere ontwikkeling van de klassieke Plasmid Mini-extractiekit, die gebaseerd is op een alkalische lysis en binding van het plasmide aan het spinfilter. De bindingscapaciteit van het spinfilter is, vergeleken met gebruikelijke plasmide-mini-kits, aanzienlijk verhoogd (ca. 80 μg plasmide-DNA) en maakt nu een plasmide-extractie mogelijk uit 0,5-15 ml bacteriecultuur. Omdat de filterafmetingen overeenkomen met de gebruikelijke miniprep-spinfilters, wijkt de toepassing slechts licht af van andere plasmide-mini-kits. Het grootste verschil is de voorafgaande handige lysis in een 15 ml-buis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVoorbereiding met het bekende mini-spincolumn-systeem\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eSnel, eenvoudig en efficiënt\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVoorbereiding uit 0,5-15 ml bacteriecultuur\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVoorbereiding binnen ca. 20 minuten\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eOpbrengst 40-60 μg (gemiddeld per 15 ml bacteriecultuur).\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eDe elutie, die resulteert in hoog geconcentreerd zuiver DNA in 100 μl, vindt vervolgens plaats in een 1,5 ml-buis, zoals in standaard mini-kits. Het geïsoleerde DNA is vrij van RNA- of eiwitcontaminatie en kan direct worden gebruikt voor alle standaard downstream-toepassingen zoals restrictievertering, sequencing, \u003ci\u003ein vitro\u003c\/i\u003e transcriptie, transfectie enz.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDe kit bevat:\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eResuspendingebuffer RB, Lysebuffer LS, Neutralisatiebuffer NB, Wasbuffer PA, Wasmiddel PB, Elutiebuffer XEB, spincolumns, opvangbuizen, elutiebuizen, gedetailleerde gebruiksaanwijzing.De inhoud van deze kit kan niet apart worden gekocht.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- DeliveryText --\u003e\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnische informatie\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eBasis materiaal\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tBacteriën en gist \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eHoeveelheid materiaal\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t0,5 - 15 ml \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eVoorbereidingstijd\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tca. 20 min \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eVersie\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tMini prep \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eAutomatisch gebruik\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tnee \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eElutievolume\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t50-100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eTypische opbrengst\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t\u003c20 μg voor 2 ml, \u003c80 μg voor 15 ml \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eTypische zuiverheid (A260\/A280)\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\t1,7-2,0 \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n\u003ca download=\"\" href=\"https:\/\/www.carlroth.com\/medias\/BA-8546-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w1ODQxOTV8YXBwbGljYXRpb24vcGRmfGFEWXhMMmhqWWk4NU1USTJNVFk1TWpFMU1EQTJMMEpCWHpnMU5EWmZSVTR1Y0dSbXxmYzU1NWNhOTNkMDUxOGZlMTYyYTMyMDhhYzJkNWIxYWMzNGFiOGM0YWQ2MDU0YWU5MmM4NjdhMzQ4ZDE3NTE0\" rel=\"noopener noreferrer\" style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\" target=\"_blank\"\u003e\n\u003csvg aria-hidden=\"true\" style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"\u003e\n\u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n\u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n\u003ctext fill=\"currentColor\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" text-anchor=\"middle\" x=\"192\" y=\"380\"\u003ePDF\u003c\/text\u003e\n\u003c\/svg\u003e\n    Technische documentatie (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"1 stuk \/ 1 stuk","offer_id":56173305102668,"sku":"8546.3","price":471.95,"currency_code":"EUR","in_stock":true},{"title":"6+ stuks \/ 6+ stuks","offer_id":56173305135436,"sku":"8546.3","price":448.35,"currency_code":"EUR","in_stock":true},{"title":"24+ stuks \/ 24+ stuks","offer_id":56173305168204,"sku":"8546.3","price":424.76,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/8546-1000Wx1000H_c6e5c9ef-c910-4f47-920d-5d808d9c45c6.jpg?v=1766443615","url":"https:\/\/www.luminixhealth.com\/nl-nl\/products\/roti-prep-plasmid-mini-xl-8546","provider":"Luminix Health","version":"1.0","type":"link"}