anti-Keratin Type II mouse, monoclonal, Ks pan1-8, 0.2 ml, plastic

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anti-Keratin Type II mouse, monoclonal, Ks pan₁-8, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

Ks pan1-8 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors. Polypeptides reacting: Mr 52,500-Mr 68,000 keratins (type II keratins K1-K8; formerly also designated cytokeratins 1-8) of human epithelial cells. Tumors specifically detected: all epithelium-derived neoplasms. Reactivity on cultured cell lines MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12.

Application

Immunocytochemistry (ICC): Assay dependentImmunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, microwave treatment recommended)Western Blot (WB): 1:500 (0.1 μg/ml)

Western blot analysis of HeLa lysate with anti-Keratin Type II antibody. Western blot analysis was performed on 20 μg HeLa lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Keratin TYpe II mouse monoclonal, Ks pan1-8 (Cat. No. 690006) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

IHC of human HNSCC tissue (courtesy of J.Heß, University Hospital Heidelberg)

IHC of mouse tongue (courtesy of J.Heß, University Hospital Heidelberg)

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IHC WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	mouse human
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG2a
Clone	Ks pan1-8
Target	Cytoskeletal proteins from cultured human MCF-7 cells

Technical Documentation (PDF)

anti-Keratin Type II mouse, monoclonal, Ks pan1-8, 0.2 ml, plastic

Description