{"product_id":"roti-garose-his-uncharged-hpbeads-0839","title":"ROTI®Garose-His uncharged HPBeads, for biochemistry, 25 ml, plastic","description":"\u003cp\u003eROTI®Garose-His uncharged HPBeads, for biochemistry, Density (D) 0,85 to 0,95 g\/cm³, Flash point (flp) \u0026gt;50 °C, Boiling point (bp) \u0026gt;80 °C, Storage temp. +4 °C, UN-Nr. 1170\u003c\/p\u003e\n\u003c!-- CARLROTH_PRODUCT_DETAILS_V7 --\u003e\n\u003cdiv class=\"tabbody ProductDetailsComponent\"\u003e\n\u003cdiv class=\"row\" style=\"display:flex; flex-wrap:wrap; width:100%; max-width:100%; margin:10px 0; gap:4px;\"\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 45%; max-width:45%; box-sizing:border-box; margin:10px 0; margin-left:-2.5%; \"\u003e\n\n\n\u003c!--superDetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eImmobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His \u003ci\u003euncharged\u003c\/i\u003e HPBeads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eEasy elution and regeneration\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eVery low metal leaching\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eCompatible with denaturing reagents\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe matrix of ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His \u003ci\u003euncharged \u003c\/i\u003eHPBeads consists of crosslinked and beaded 6 % agarose, IDA-conjugated. In one step, the beads can be loaded safely and rapidly with all standard bivalent metal cations (Ni\u003csup\u003e2+\u003c\/sup\u003e, Co\u003csup\u003e2+\u003c\/sup\u003e, Zn\u003csup\u003e2+\u003c\/sup\u003e, Cu\u003csup\u003e2+\u003c\/sup\u003e). ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His \u003ci\u003euncharged\u003c\/i\u003e HPBeads result in eluates with considerably low metal contamination.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eThe tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His uncharged HPBeads may repeatedly be regenerated, making them very cost-effective.The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003c!-- OtherDetailElements --\u003e\n\u003c!-- Note --\u003e\n\n\n\u003c!-- Base product name --\u003e\n\n\u003c!-- DetailElements --\u003e\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eUncharged IDA-agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-\/matrix volume.In ROTI\u003csup\u003e®\u003c\/sup\u003eGarose-His \u003ci\u003euncharged\u003c\/i\u003e HPBeads, the advantages of the efficient metal ion\/His binding have been combined with a bead technology allowing a very rapid flow rate.50 % bead suspension in 20 % ethanol.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-container\"\u003e\n\n\u003cdiv\u003e\u003cul style=\"margin:10px 0; padding-left:20px; \"\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eRapid one-step purification of His-tagged proteins from total lysates \u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003eHigh binding capacity for standard me\u003csup\u003e2+\u003c\/sup\u003e ions under rapid one-step loading\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eFor batch mode, gravity flow, MPLC und FPLC\u003c\/b\u003e\u003c\/li\u003e\n\u003cli style=\"margin:6px 0; word-wrap:break-word; overflow-wrap:anywhere; \"\u003e\u003cb\u003eRecommended for big matrix volumes\u003c\/b\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eSuitable for\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eSuitable for MPLC and FPLC, or if His-tag proteins shall be isolated either in particularly short time or in big amounts.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\u003cdiv class=\"item\" style=\"word-wrap:break-word; overflow-wrap:anywhere; hyphens:auto; \"\u003e\n\u003cdiv class=\"details-container\"\u003e\n\u003cdiv class=\"paragraph-title\"\u003eDirections for use\u003c\/div\u003e\n\u003cp style=\"font-size:0.95em; line-height:1.5; margin:12px 0; word-wrap:break-word; overflow-wrap:anywhere; white-space:normal; \"\u003eMay be autoclaved at 121 °C for 30 mins.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\n\u003c\/div\u003e\n\n\n\n\u003c!-- DeliveryText --\u003e\n\n\u003c!-- printCatalogLink --\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-xs-12 col-md-6\" style=\"flex:0 0 55%; max-width:55%; box-sizing:border-box; margin:10px 0; margin-left:-3%; \"\u003e\n\u003cdiv class=\"product-classifications\"\u003e\n\u003cdiv class=\"headline\" style=\"font-weight:bold; font-size:1.2em; margin-bottom:10px;\"\u003eTechnical Information\u003c\/div\u003e\n\u003ctable class=\"table\" style=\"; table-layout:fixed; word-break:normal; overflow-wrap:break-word; width:100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd class=\"attrib\" style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-weight:bold; font-size:1em; white-space:normal; overflow-wrap:break-word; hyphens:auto; word-break:normal; \"\u003eInstructions for use, addition\u003c\/td\u003e\n\u003ctd style=\"padding:8px 6px; vertical-align:top; box-sizing:border-box; font-size:0.92em; white-space:normal; word-break:normal; overflow-wrap:break-word; hyphens:auto; \"\u003e\n\t\t\t\t\t\t\t\t\tBead suspension \u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cdiv style=\"margin-top:1.5rem;\"\u003e\n  \u003ca href=\"https:\/\/www.carlroth.com\/medias\/BA-0839-EN.pdf?context=bWFzdGVyfGluc3RydWN0aW9uc3w0Mzg4NTh8YXBwbGljYXRpb24vcGRmfGFXNXpkSEoxWTNScGIyNXpMMmd3T1M5b09XVXZPVEF6Tnpjek9EazJOekEzTUM1d1pHWXwxN2EzNmE1Nzk1MmYyMDkyOWQ0Zjg4Mzg4ZjJiYzEzNzkyZGExYjUyZmQ3ZTY2Zjc5MzMwOWVjZDU2MGEyZWQ4\" target=\"_blank\" rel=\"noopener noreferrer\" download style=\"display:inline-flex; align-items:center; gap:.65rem; text-decoration:none; color:#000; font-weight:600; font-size:1rem;\"\u003e\n    \u003csvg style=\"width:1.65rem;height:1.65rem;color:#dc2626;flex-shrink:0;\" viewbox=\"0 0 384 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" aria-hidden=\"true\"\u003e\n      \u003cpath d=\"M64 0C28.7 0 0 28.7 0 64V448c0 35.3 28.7 64 64 64H320c35.3 0 64-28.7 64-64V160H256c-17.7 0-32-14.3-32-32V0H64z\" fill=\"#ffffff\" stroke=\"currentColor\" stroke-width=\"32\"\u003e\u003c\/path\u003e\n      \u003cpath d=\"M256 0V128H384L256 0z\" fill=\"currentColor\"\u003e\u003c\/path\u003e\n      \u003ctext x=\"192\" y=\"380\" font-family=\"Arial, Helvetica, sans-serif\" font-size=\"110\" font-weight=\"bold\" fill=\"currentColor\" text-anchor=\"middle\"\u003ePDF\u003c\/text\u003e\n    \u003c\/svg\u003e\n    Technical Documentation (PDF)\n  \u003c\/a\u003e\n\u003c\/div\u003e","brand":"Carl Roth","offers":[{"title":"25 ml \/ 1 piece","offer_id":56112606675276,"sku":"0839.1","price":385.95,"currency_code":"EUR","in_stock":true},{"title":"25 ml \/ 6+ pieces","offer_id":56112606708044,"sku":"0839.1","price":366.65,"currency_code":"EUR","in_stock":true},{"title":"25 ml \/ 24+ pieces","offer_id":56112606740812,"sku":"0839.1","price":347.36,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 1 piece","offer_id":56112606773580,"sku":"0839.2","price":1069.65,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 6+ pieces","offer_id":56112606806348,"sku":"0839.2","price":1016.17,"currency_code":"EUR","in_stock":true},{"title":"100 ml \/ 24+ pieces","offer_id":56112606839116,"sku":"0839.2","price":962.69,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0955\/4827\/7068\/files\/0839-1000Wx1000H.jpg?v=1766399071","url":"https:\/\/www.luminixhealth.com\/en-nl\/products\/roti-garose-his-uncharged-hpbeads-0839","provider":"Luminix Health","version":"1.0","type":"link"}