anti-HA-tag mouse, monoclonal, 12CA5, after dissolv. in 100 µl PBS, 100 µl - Luminix Health
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anti-HA-tag mouse, monoclonal, 12CA5, after dissolv. in 100 µl PBS, 100 µl - Luminix Health

anti-HA-tag mouse, monoclonal, 12CA5, after dissolv. in 100 µl PBS, 100 µl

SKU: 1NN7.1
Volume: 25 µg
Value Packs: 1 piece
Price:
€123,65

Description

anti-HA-tag mouse, monoclonal, 12CA5, after dissolv. in 100 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) 100 °C, Storage temp. +4 °C

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

  • Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
  • High affinity to the respective protein tag
  • Robust & highly specific detection of the fusion protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

The monoclonal 12CA5 antibody recognizes the HA-tag (hemagglutinin tag). The HA-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Application

Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)

Western blot analysis of E. coli lysate containing HA-tagged protein with anti-HA-tag antibody. Western blot analysis was performed on 10 ng or 5 ng of E. coli lysate containing HA-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5 % dry milk in PBST for 1 h at RT. The primary antibody anti-HA-tag mouse monoclonal, 12CA5 (Cat. No. 910HAL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Not a medical device / Not an IVD product
Technical Information
Application WB 
Host species mouse 
Clonality monoclonal 
Purification Affinity chromatography 
Conjugate uncojugated 
Isotype IgG2b 
Clone 12CA5 
Target HA-Tag 

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