anti-Plakoglobin mouse, monoclonal PG 5.1, 0.2 ml, plastic - Luminix Health
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anti-Plakoglobin mouse, monoclonal PG 5.1, 0.2 ml, plastic - Luminix Health

anti-Plakoglobin mouse, monoclonal PG 5.1, 0.2 ml, plastic

SKU: 1NNC.1
Volume: 0.2 ml
Value Packs: 1 piece
Price:
€109,15

Description

anti-Plakoglobin mouse, monoclonal PG 5.1, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: desmosomes of epithelial and myocardial cells (incl. cultured cells); zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide reacting: Mr 83,000 Plakoglobin, 'band 5' polypeptide of intercellular adhering junctions (identical to gamma-catenin). Reactivity on cultured cell lines: cell cultures forming monolayers.

Application

Immunocytochemistry (ICC): 1:5 (10 μg/ml)Immunohistochemistry (IHC) - frozen: 1:25-1:100 (0.5-2 μg/ml)Immunohistochemistry (IHC) - paraffin: 1:25-1:100 (0.5-2 μg/ml, microwave treatment recommended)Western Blot (WB): 1:500-1:2,000 (0.025-0.1 μg/ml)

IHC of human oral mucosa (courtesy of J.Heß, University Hospital Heidelberg)
IHC of rat tongue (courtesy of J.Heß, University Hospital Heidelberg)
Western blot analysis of Ins-1 lysate and with anti-Plakoglobin antibody. Western blot analysis was performed on 20 μg rat Ins-1 lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Plakoglobin mouse monoclonal, PG 5.1 (Cat. No. 690005) was diluted in blocking buffer (antibody concentration 25 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 200 ng/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Not a medical device / Not an IVD product
Technical Information
Application ICC/IF IHC WB 
Host species mouse 
Clonality monoclonal 
Reactivity antibody cattle dog human mouse 
Purification Affinity chromatography 
Conjugate uncojugated 
Concentration 50 µg/ml 
Isotype IgG2b 
Clone PG 5.1 
Target plakoglobin, 'band 5' protein 

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