Description
Dulbecco's Phosphate Buffered Saline with calcium and magnesium is an isotonic buffer solution formulated to keep cell culture media within the physiological pH range of 7,2–7,6. It is widely used as a balanced saline or wash buffer for cells and tissues, helping maintain osmotic balance during handling, rinsing and short-term incubation steps. The formulation is modified to include both calcium and magnesium, supporting applications where these divalent cations are required for cell adhesion or physiological function. This DPBS from Sartorius serves routine cell-culture workflows, sample preparation and gentle washing of monolayers or tissue pieces without introducing unwanted osmotic stress. Supplied as a ready laboratory unit with a net weight of 0,500 kg, it fits standard media-handling practices in research and bioprocessing laboratories. The buffer system is intended for maintaining physiological conditions rather than as a complete growth medium, making it a practical companion reagent alongside culture media and dissociation solutions.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack size: 1 pc
- Storage temperature: 2–8 °C
- Type: Isotonic DPBS buffer
- Composition: With calcium and magnesium
- pH range: 7,2–7,6
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Questions about Dulbecco's PBS 500ML
What is this article?
Dulbecco's PBS 500ML is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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