Description
Roti®Nylon Plus Reprobe, pos. charged, 5x 30.5 x 30.5 cm, 0.45 µm
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The optimal membrane for DNA/RNA transfer. ROTI®Nylon Plus Reprobe is a cationic, supported nylon 6.6 membrane specially developed for multiple reprobings. The membrane has an extremely high tensile strength. The inherent positively charged membrane surface guarantees fast and efficient binding of DNA and RNA. After 12 rounds of reprobing, ROTI®Nylon Plus Reprobe has a lower background and a higher signal. Steam sterilisation is possible.Also available as practical pre-cuts in common gel sizes!Fixation of the nucleic acids on the membrane can be achieved by UV irradiation. No fixation is required for alkaline transfer conditions. Under these conditions, the negatively charged DNA forms a very stable complex with the positively charged ROTI®Nylon Plus Reprobe membrane.
- Southern and Northern blotting
- Alkaline blotting
- Multiple reprobings
| Dimensions | 30.5 × 30.5 cm |
| Pore size | 0,45 µm |
| Bond | hydrophile |
| Load | positive |
| Thickness | 120-190 µm |
| Immobilisation | not required |
| Binding capacity | 450 µg/cm² |